LC-MS/MS method for hepcidin-25 measurement in human and mouse serum: clinical and research implications in iron disorders

Clinical Chemistry and Laboratory Medicine : CCLM
Thibaud LefebvreZoubida Karim

Abstract

The peptide hepcidin plays a central role in regulating dietary iron absorption and body iron distribution. This 25-amino acid hormone is produced and secreted predominantly by hepatocytes. Hepcidin has been suggested as a promising diagnostic marker for iron-related disorders. However, its accurate quantification for clinical use remains so far challenging. In this report we describe a highly specific and quantitative serum hepcidin method using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The analytical validation included the determination of the limit of detection, of quantification, repeatability, reproducibility and linearity. This assay was developed for human and mouse hepcidin. The human assay was performed on serum patients with unexplained microcytic anemia. We applied our LC-MS/MS method for quantifying hepcidin-1 in mouse in various conditions: inflammation, hemolytic anemia, Hamp-1, Hjv and Hfe KO mice. We show that the LC-MS/MS is suitable for accurate determination of hepcidin-25 in clinical samples, thereby representing a useful tool for the clinical diagnosis and follow-up of iron-related diseases. In mouse, a strong correlation between hepatic Hamp-1 mRNA expression and serum hepcid...Continue Reading

References

Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·G NicolasS Vaulont
Nov 8, 2003·Blood·Dan-Qing LouSophie Vaulont
Apr 1, 2006·Blood·Jeanne-Claire Lesbordes-BrionSophie Vaulont
Sep 6, 2006·Gastroenterology·Soumeya BekriYannick Le Marchand-Brustel
Aug 12, 2008·Blood·Tomas GanzMark Westerman
Mar 5, 2009·Journal of Pharmacological and Toxicological Methods·Hongyan LiBarbra J Sasu
Jun 26, 2009·The Journal of Biological Chemistry·John B JordanBarbra J Sasu
Sep 30, 2009·Seminars in Hematology·Tomas Ganz, Elizabeta Nemeth
Dec 8, 2010·Cardiovascular Drugs and Therapy·Kristopher S LyonsMark T Harbinson
Mar 17, 2011·PloS One·Harold TjalsmaDorine W Swinkels
Aug 27, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sun-Il HwangHerbert L Bonkovsky
Oct 13, 2011·Clinical Chemistry·Joyce J C KrootDorine W Swinkels
Jan 10, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Outi ItkonenEsa Hämäläinen
Aug 14, 2012·American Journal of Hematology·Joyce J C KrootDorine W Swinkels
Apr 26, 2013·Kidney International·Boualem MoulouelZoubida Karim
May 7, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fleur WolffFrédéric Cotton
Jun 5, 2013·Haematologica·Luigia De FalcoCarole Beaumont
Aug 7, 2013·Nutrients·Andrea U Steinbicker, Martina U Muckenthaler
May 31, 2014·Metallomics : Integrated Biometal Science·T KonzS Vaulont

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Citations

Jan 21, 2017·Bioanalysis·Constance DelabyChristophe Hirtz
May 5, 2017·Metallomics : Integrated Biometal Science·Dominic J Hare
Jul 12, 2019·Science Translational Medicine·Sabrina BonduMichaëla Fontenay
Nov 26, 2015·Critical Care Medicine·Sigismond LasockiClementine Schilte
May 16, 2019·Scientific Reports·Ahmed MoghiebCharles Ansong
Apr 14, 2016·The Journal of Biological Chemistry·Ningning ZhaoAn-Sheng Zhang
Sep 20, 2017·The Journal of Biological Chemistry·Mastura WahediAn-Sheng Zhang
Aug 20, 2019·World Journal of Clinical Cases·Raêd DaherZoubida Karim
Apr 19, 2018·Analytical and Bioanalytical Chemistry·Ioana M AbbasMichael G Weller
Oct 2, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Ellis T AuneDorine W Swinkels
Oct 12, 2019·Biochemical and Biophysical Research Communications·Thibaud LefebvreCécile Ged
Jul 17, 2021·Journal of Proteome Research·Rachel E ForemanRichard G Kay

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