LC-NMR: a new tool to expedite the dereplication and identification of natural products

Journal of Industrial Microbiology & Biotechnology
S C BobzinT P Kasten

Abstract

The rapid identification of known or undesirable compounds from natural products extracts - "dereplication" - is an important step in an efficiently run natural products discovery program. Dereplication strategies use analytical techniques and database searching to determine the identity of an active compound at the earliest possible stage in the discovery process. In the past few years, advances in technology have allowed the development of tandem analytical techniques such as liquid chromatography mass spectrometry (LC-MS), LC-MS-MS, liquid chromatography nuclear magnetic resonance (LC-NMR), and LC-NMR-MS. LC-NMR, despite its lower sensitivity as compared to LC-MS, provides a powerful tool for rapid identification of known compounds and identification of structure classes of novel compounds. LC-NMR is especially useful in instances where the data from LC-MS are incomplete or do not allow confident identification of the active component of a sample. LC-NMR has been used to identify the marine alkaloid aaptamine as the active component in an extract of the sponge Aaptos sp. This extract had been identified as an enzyme inhibitor by a high throughput screening (HTS) effort. Isolated aaptamine exhibited an IC(50)=120 µM against t...Continue Reading

Citations

Jan 29, 2009·Journal of Separation Science·Daniel Anthony Dias, Sylvia Urban
Jul 8, 2010·The Journal of Antibiotics·Xiangyang LiuLixin Zhang
Oct 6, 2010·Natural Product Reports·Elizabeth Jane AshforthLixin Zhang
Nov 17, 2011·Antonie van Leeuwenhoek·Xueting LiuLixin Zhang
Jan 21, 2014·Analytical and Bioanalytical Chemistry·Andreas KlitgaardKristian Fog Nielsen
Feb 25, 2003·Phytochemical Analysis : PCA·William N SetzerWilliam A Haber
May 1, 2019·Frontiers in Microbiology·Momen M LotfyNoha M Gamaleldin
Jan 17, 2020·Metabolites·Mohammed S HifnawyUsama Ramadan Abdelmohsen
Apr 9, 2015·Natural Product Reports·Susana P Gaudêncio, Florbela Pereira
Jun 7, 2014·Marine Drugs·Lynsey MacintyreRuAngelie Edrada-Ebel
Mar 15, 2011·Progress in Nuclear Magnetic Resonance Spectroscopy·André J SimpsonMyrna J Simpson
Oct 27, 2011·Journal of Natural Products·Kristian F NielsenThomas O Larsen
Aug 15, 2018·Journal of Natural Products·Fei LiuShi-Shan Yu
Jan 5, 2002·Journal of Natural Products·J BradshawP J Sidebottom
Dec 29, 2004·Journal of Natural Products·Mark S Butler

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