LC of sulfonamide residues in poultry muscle and eggs extracts using fluorescence pre-column derivatization and monolithic silica column

Journal of Separation Science
Anastasia Zotou, Christina Vasiliadou

Abstract

A new, rapid, sensitive and selective HPLC method with fluorescence detection is described for the simultaneous determination of 12 sulfonamides, in the presence of putrescine as internal standard, after pre-column derivatization with fluorescamine. The drugs were separated on a Chromolith Performance RP-18 column (100x4.6 mm), using a gradient elution with a binary mobile phase of methanol/0.05 M acetate buffer (pH 3.4). Linearity of derivatization was obtained for concentrations from 3.0 to 300 microg/L in standard solutions. The whole procedure was evaluated and fully validated, according to the European Union Decision 2002/657/EC, for the determination of sulfonamides in turkey muscle and hen eggs following SPE. The LODs varied from 2 to 17 microg/kg in turkey and 2 to 15 microg/kg in egg samples. The average recoveries ranged between 96.9-108.6% in turkey muscle and 96.0-108.4% in egg samples, respectively.

References

Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·R J Maxwell, A R Lightfield
Jul 20, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J A TarbinG Shearer
Mar 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·M T CombsL T Taylor
Apr 19, 2002·Food Additives and Contaminants·B Roudaut, M Garnier
Jun 8, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David N HellerMichael H Thomas
Oct 4, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·P T VallanoB K Matuszewski
Nov 28, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiliang WangDingren Bi
Aug 22, 2009·Toxicology Mechanisms and Methods·Arkadiusz Szymański

Citations

Sep 24, 2013·Food Chemistry·Natalia Arroyo-ManzanaresAna M García-Campaña
May 24, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mingyuan ShaoYong Yu
Jun 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martha MaggiraVictoria F Samanidou
Feb 20, 2017·Environmental Science and Pollution Research International·Yang XuLan Ding

Related Concepts

High Pressure Liquid Chromatography Procedure
Drug Residues
Egg Food Product
Food Contamination
Meat
Fowls, Domestic
Quso G-32
Fluorescence Spectroscopy
Sulfonamides
Meleagridinae

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