LC/ESI/MS method development for the analysis of hepatotoxic cyclic peptide microcystins in animal tissues

Toxicon : Official Journal of the International Society on Toxinology
Jennifer L Ott, W W Carmichael

Abstract

Microcystins (MCYSTs) are a family of related cyclic heptapeptides produced by several genera and species of blue-green algae (cyanobacteria). MCYSTs are potent and specific inhibitors of the serine threonine family of protein phosphatases, especially PP1 and PP2A. MCYSTs inhibit a liver's protein phosphatase by forming a covalent linkage between MCYSTs' Mdha residue and the phosphatase's cysteine residue. Due to the covalent linkage, analysis of MCYSTs in animal tissues has been limited to determination of unbound MCYST concentration. The MMPB (2-methyl-3-methoxy-4-phenylbutyric acid) oxidation procedure allows for the detection of total MCYST burden by releasing the carboxylic acid MMPB from MCYST's Adda amino acid. An internal standard 4-phenylbutyric acid (4PB) accounts for losses during the method. LC/MS conditions were developed using a ThermoFinnigan LCQDuo ion trap in negative electrospray ionization (ESI). Since both compounds produce the [M-H](-) ion, analysis occurs in selected ion monitoring (SIM) mode for both MMPB (m/z 207.1) and 4PB (m/z 163.1). Complete oxidation of MCYST-LR in liver tissues occurs in 3h. A solid phase extraction (SPE) cartridge removes MMPB and 4PB from the oxidant solution. The process efficie...Continue Reading

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Citations

Jan 22, 2014·Archives of Environmental Contamination and Toxicology·Amina AmraniNoureddine Bouaïcha
Apr 21, 2006·Saline Systems·Wayne W Carmichael, RenHui Li
Jan 2, 2009·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·José C Martins, Vítor M Vasconcelos
Apr 7, 2010·Toxicon : Official Journal of the International Society on Toxinology·Felipe Augusto DörrSandra Maria Feliciano de Oliveira e Azevedo
Mar 6, 2010·Chemosphere·Heng-Feng MiaoWen-Quan Ruan
Jan 15, 2010·Ecotoxicology and Environmental Safety·Juliette L SmithGregory L Boyer
Dec 10, 2008·Toxicon : Official Journal of the International Society on Toxinology·Juliette L Smith, Gregory L Boyer
Feb 1, 2008·Ecotoxicology and Environmental Safety·Hichem NasriNoureddine Bouaïcha
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Aug 19, 2007·Environmental Toxicology·E D HilbornS M F O Azevedo
Jun 28, 2011·Toxicon : Official Journal of the International Society on Toxinology·Hung-Kai YenPao-Chi Liao
Nov 30, 2011·Analytica Chimica Acta·Xingqiang WuJingshuang Liu
Sep 6, 2006·Toxicon : Official Journal of the International Society on Toxinology·Moucun YuanElizabeth D Hilborn
Jul 30, 2015·Toxicon : Official Journal of the International Society on Toxinology·Amanda J Foss, Mark T Aubel
Jul 28, 2016·Journal of Hazardous Materials·Sevasti-Kiriaki ZervouAnastasia Hiskia
Jun 28, 2016·Chemistry : a European Journal·Ivan ZemskovValentin Wittmann
Jan 25, 2012·International Journal of Molecular Sciences·José Carlos MartinsVitor Vasconcelos
Sep 2, 2017·Toxicon : Official Journal of the International Society on Toxinology·Laura RosenblumSteven C Wendelken
Aug 14, 2020·The Science of the Total Environment·Maryam Tabatabaei AnarakiXavier Ortiz Almirall

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