LCM-seq reveals the crucial role of LsSOC1 in heat-promoted bolting of lettuce (Lactuca sativa L.)

The Plant Journal : for Cell and Molecular Biology
Zijing ChenQian Wang

Abstract

Lettuce (Lactuca sativa L.) is one of the most economically important vegetables. The floral transition in lettuce is accelerated under high temperatures, which can significantly decrease yields. However, the molecular mechanism underlying the floral transition in lettuce is poorly known. Using laser capture microdissection coupled with RNA sequencing, we isolated shoot apical meristem cells from the bolting-sensitive lettuce line S39 at four critical stages of development. Subsequently, we screened specifically for the flowering-related gene LsSOC1 during the floral transition through comparative transcriptomic analysis. Molecular biology, developmental biology, and biochemical tools were combined to investigate the biological function of LsSOC1 in lettuce. LsSOC1 knockdown by RNA interference resulted in a significant delay in the timing of bolting and insensitivity to high temperature, which indicated that LsSOC1 functions as an activator during heat-promoted bolting in lettuce. We determined that two heat shock transcription factors, HsfA1e and HsfA4c, bound to the promoter of LsSOC1 to confirm that LsSOC1 played an important role in heat-promoted bolting. This study indicates that LsSOC1 plays a crucial role in the heat-pr...Continue Reading

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Citations

Jan 27, 2019·Plant, Cell & Environment·Kang NingQian Wang
Mar 26, 2021·Frontiers in Plant Science·Rongkui HanRichard W Michelmore
Jul 11, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rongkui HanRichard Michelmore
Jul 2, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Leah RosentalIvan Simko

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