LCMS and GCMS for the screening of alkaloids in natural and in vitro extracts of Leucojum aestivum

Journal of Natural Products
Agata PtakDominique Laurain-Mattar

Abstract

HPLC coupled to a mass spectrometer (MS) was used for the analysis of galanthamine and lycorine in natural extracts of Leucojum aestivum and in their in vitro cultures grown with a precursor (ACC), inhibitors (AgNO(3), STS), or an absorber (KMnO(4)) of ethylene. The maximum galanthamine (0.002%) and lycorine (0.02%) concentrations in tissue cultures were obtained in the presence of KMnO(4). GCMS was used to investigate underivatized alkaloid mixtures from L. aestivum. Seven alkaloids were identified in in vivo bulbs. KMnO(4) led to the highest diversity of alkaloids in tissue culture extracts.

References

Feb 1, 1995·Planta medica·B WenigerR Anton
Jun 18, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Nguyen Thi Ngoc TramSimeon S Popov
Sep 24, 2004·Planta medica·László SzlávikJudit Hohmann
May 11, 2005·Phytochemical Analysis : PCA·Strahil BerkovMarina Stanilova
Mar 22, 2008·Bioorganic & Medicinal Chemistry Letters·Strahil BerkovJaume Bastida

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Citations

Jan 14, 2011·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yoshimasa Kasahara
Sep 11, 2009·Phytochemical Analysis : PCA·Andrea LubbeRobert Verpoorte
Jun 5, 2014·TheScientificWorldJournal·Sreeramanan SubramaniamVikneswaran Murugaiyah
Apr 19, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dinar S C WahyuniKirsten Leiss
Apr 8, 2011·Natural Product Reports·Zhong Jin
Jan 1, 2013·Science China. Chemistry·ZhiFei CaoQuanSheng Zhou
Oct 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vasil GeorgievAtanas Pavlov
Aug 17, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mridul RoyJing Liu
May 7, 2019·Journal of Pharmaceutical and Biomedical Analysis·Ameneh TarakemehDominique Laurain-Mattar

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