Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis

Frontiers in Oncology
Tao JinYiquan Ke

Abstract

Background: Glioma is the most common malignant tumor of the central nervous system, and often displays invasive growth. Recently, circular RNA (circRNA), which is a novel non-coding type of RNA, has been shown to play a vital role in glioma tumorigenesis. However, the functions and mechanism of lipocalin-2 (Lcn2)-derived circular RNA (hsa_circ_0088732) in glioma progression remain unclear. Methods: We evaluated hsa_circ_0088732 expression by fluorescence in situ hybridization (FISH), Sanger sequencing, and PCR assays. Cell apoptosis was evaluated by flow cytometry and Hoechst 33258 staining. Transwell migration and invasion assays were performed to measure cell metastasis and viability. In addition, the target miRNA of hsa_circ_0088732 and the target gene of miR-661 were predicted by a bioinformatics analysis, and the interactions were verified by dual-luciferase reporter assays. RAB3D expression was analyzed by an immunochemistry assay, and E-cadherin, N-cadherin, and vimentin protein expression were examined by western blot assays. A mouse xenograft model was developed and used to analyze the effects of hsa_circ_0088732 on glioma growth in vivo. Results: We verified that hsa_circ_0088732 is circular and highly expressed in g...Continue Reading

References

Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J M LarkinM A McNiven
Aug 10, 2002·Histology and Histopathology·A L MillarM H Zheng
Feb 1, 2003·Lancet·Anthony BehinJean-Yves Delattre
Jun 18, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jan TheysMarc Vooijs
Jan 4, 2012·Journal of Cellular Biochemistry·Roxana S RedisGeorge A Calin
Mar 1, 2013·Nature·Thomas B HansenJørgen Kjems
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Apr 11, 2014·The Oncologist·Jessica KentwellStan B Sidhu
Sep 23, 2014·Molecular Cell·Reut Ashwal-FlussSebastian Kadener
Jan 31, 2015·Seminars in Liver Disease·Ashley M Mohr, Justin L Mott
Jan 19, 2016·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Maximilian NiyaziClaus Belka
Apr 24, 2016·Mathematical Biosciences and Engineering : MBE·Christian EngwerChristina Surulescu
Sep 7, 2016·Molecular Carcinogenesis·Huda H Al-Khalaf, Abdelilah Aboussekhra
Oct 13, 2016·Frontiers in Physiology·Anastasia AsimakopoulouRalf Weiskirchen
Nov 24, 2016·Acta neurochirurgica·Joeky T SendersMarike L D Broekman
Jan 25, 2017·Brain Tumor Pathology·Toru Kondo
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
Feb 19, 2017·Trends in Endocrinology and Metabolism : TEM·Alexander R MoschenHerbert Tilg
Mar 28, 2017·Cancer Letters·Jun HeYongsheng Li
Mar 28, 2017·Pathology, Research and Practice·Jun-Tao YaoKe-Jun Nan
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Peili ZhangLei Jiang
May 5, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jin ZhangLiangzhang Sun
May 26, 2017·Molecular Cancer·Shujuan MengMinghua Wu
Jun 8, 2017·International Journal of Molecular Sciences·Pui-Wah Choi, Shu-Wing Ng
Jun 20, 2017·Annual Review of Nutrition·Xia XiaoMatam Vijay-Kumar
Jul 12, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ben-Gang WangQian Xu
Sep 15, 2017·Journal of the National Cancer Institute·Yibing YangNu Zhang

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Citations

Nov 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yuan LuRonald Walter
May 14, 2021·Journal of Cellular Biochemistry·Robert D Raffaniello
May 18, 2021·Frontiers in Genetics·Liang CenHongjuan Cui

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Methods Mentioned

BETA
GTPases
GTPase
biopsies
Assay
Transfection
PCR
electrophoresis
protein assay
X-ray
Flow Cytometry

Software Mentioned

TargetScan

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