LDL accelerates monocyte to macrophage differentiation: Effects on adhesion and anoikis

Atherosclerosis
R EscateL Badimon

Abstract

High LDL triggers dyslipidemia and atherosclerosis, a chronic inflammatory disease with participation of the innate immunity system. Monocytes are recruited to areas of LDL-induced endothelial damage and initiate differentiation. This study was aimed to investigate the effects of LDL on the early transitional stages of monocyte differentiation into macrophages. Blood monocytes, isolated from healthy donors by their adhesion properties, were exposed to native-LDL (1.80 mg/mL) for 48-h. Monocyte phenotype was assessed at transcript and miRNA levels by real-time PCR. Protein-expression was determined by western-blot and flow-cytometry. CD14 time-dependently decreased in adhered monocytes, reaching a >4 fold decrease at transcript- and protein-levels after 7-days in culture when cells were already differentiated into macrophages. At 4-days differentiation, monocytes exposed to LDL reduced CD14-transcrition >1.5 fold in mRNA (p = 0.002) and 34% CD14-protein (p = 0.039), whereas increased in CD16-expression (p = 0.019). Besides, LDL induced a significant increase in integrin CD49c (α3-subunit) at mRNA (>2 fold, p = 0.008) and protein (>3 fold, p = 0.045) level and a decrease in the apoptosis-effectors CASP8 and CASP3 (p = 0.002 and p...Continue Reading

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Citations

May 3, 2016·Regenerative Engineering and Translational Medicine·Sean AllenEvan Scott
Sep 30, 2016·Thrombosis and Haemostasis·Luca LiberaleFederico Carbone
Feb 18, 2017·Current Opinion in Hematology·Lina Badimon, Maria Borrell-Pages
Nov 2, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rafael EscateLina Badimon
Feb 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rafael EscateLina Badimon
Apr 4, 2017·Cardiovascular Therapeutics·Su-Jie JiaMing Zhao
Feb 1, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rafael EscateLina Badimon
Dec 18, 2018·Frontiers in Immunology·Julia LeonhardtRegine Heller
Mar 6, 2021·Nitric Oxide : Biology and Chemistry·Ya-Ying ChangTzu-Yu Lin
Apr 16, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Susanna FiorelliSonia Eligini
Jun 3, 2021·International Journal of Molecular Sciences·Maisa Garcia-ArguinzonisTeresa Padro

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