LdtR is a master regulator of gene expression in Liberibacter asiaticus

Microbial Biotechnology
Fernando A PagliaiGraciela L Lorca

Abstract

Huanglongbing or citrus greening disease is causing devastation to the citrus industry. Liberibacter asiaticus, an obligate intracellular pathogen of citrus, is one the causative agents of the disease. Most of the knowledge about this bacterium has been deduced from the in silico exploration of its genomic sequence. L. asiaticus differentially expresses genes during its transmission from the psyllid vector, Diaphorina citri, to the plant. However, the regulatory mechanisms for the adaptation of the bacterium into either hosts remain unknown. Here we show that LdtR, a MarR family transcriptional regulator, activates or represses transcription genome-wide. We performed a double approach to identify the components of the LdtR regulon: a transcriptome analysis in both the related bacterium Liberibacter crescens and citrus-infected leaves, strengthened with an in silico prediction of LdtR regulatory sites. Our results demonstrated that LdtR controls the expression of nearly 180 genes in L. asiaticus, distributed in processes such as cell motility, cell wall biogenesis, energy production, and transcription. These results provide new evidence about the regulatory network of L. asiaticus, where the differential expression of genes from...Continue Reading

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Citations

Apr 11, 2018·PloS One·Fernando A PagliaiGraciela L Lorca
Mar 30, 2019·Molecular Plant-microbe Interactions : MPMI·Maxuel Andrade, Nian Wang
Aug 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Melanie J BarnettSharon R Long
Dec 16, 2018·Applied and Environmental Microbiology·Lei PanGraciela L Lorca
Aug 24, 2021·Frontiers in Microbiology·Marcelo L MerliClaudio F Gonzalez
Aug 26, 2021·Environmental Microbiology·Lei PanGraciela L Lorca

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Methods Mentioned

BETA
footprinting
RNA
Illumina sequencing
PCR
electrophoresis
X‐ray
RNAseq

Software Mentioned

Cutadapt
RegPredict
package K ‐ means
Blast2Go
Sickle
MicroCal Origin
DESeq2
LOGO
HTseq
R

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