PMID: 6159644Sep 1, 1980Paper

Le interferon mRNA from human fibroblasts

Proceedings of the National Academy of Sciences of the United States of America
R H PangJ Vilcek

Abstract

Human F and Le interferon can be clearly distinguished on the basis of different antigenic properties and host range. After inoculation with Newcastle disease virus (NDV), GM-258 fibroblasts produced Le as well as F interferon; in contrast, only F interferon was detectable after stimulation with poly(I) . poly(C). Polyadenylylated mRNA isolated from fibroblasts induced with poly(I) . poly(C) or NDV was injected into Xenopus laevis oocytes and the interferon activities thus produced were analyzed. Only F interferon production was demonstrable in oocytes injected with mRNA from cells induced with poly(I) . poly(C), whereas both F and Le interferons were made in oocytes injected with mRNA from NDV-induced cultures. The time course of accumulation of F and Le interferon mRNAs in NDV-induced cells corresponded to the kinetics of F and Le interferon synthesis in intact cells. The ratio of F and Le interferons made in oocytes was similar to that observed in intact GM-258 cells. F and Le interferon mRNA activities isolated from GM-258 cells could not be separated by sucrose density gradient centrifugation. However, the profile of F mRNA activity was more heterogeneous and its peak sedimented somewhat more slowly than that of Le interfe...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A HavellJ Vilcek
Jan 1, 1978·The Journal of General Virology·E A HavellJ Vilcek
Nov 1, 1979·The Journal of General Virology·A MeagerA Westerveld
Nov 17, 1975·Biochemical and Biophysical Research Communications·R H Pang, L A Phillips
Aug 1, 1978·Virology·E A HavellJ Vilcek
Oct 30, 1979·Virology·T G HayesJ Vilcek
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L CavalieriS Pestka
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalI Tamm
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L CavalieriS Pestka
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S PestkaJ Vilcek
Dec 11, 1975·Proceedings of the National Academy of Sciences of the United States of America·F H ReynoldsP M Pitha
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·Y H TanF H Ruddle
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J Vilcek, E A Havell
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J de Maeyer-GuignardL Montagnier
Jun 1, 1980·Gene·T TaniguchiM Muramatsu

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Citations

Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T TaniguchiJ Vilcek
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Ohno, T Taniguchi
Jan 1, 1982·Journal of Interferon Research·A MeagerJ Vilcek

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