Leaching of bioluminescent Escherichia coli O157:H7 from sheep and cattle faeces during simulated rainstorm events

Journal of Applied Microbiology
A P WilliamsKen Killham

Abstract

Development of a novel inoculation technique to improve the current methods of determining the leaching of Escherichia coli O157:H7 from faeces. Ruminant faeces were inoculated with a high [c. 10(7) colony forming units (CFU) g(-1)] or low (c. 10(4) CFU g(-1)) load of a lux-marked strain of E. coli O157:H7 via injection, and subjected to four simulated heavy rainfall events. The population density and metabolic activity of E. coli O157:H7 recovered within the leachate was determined following each simulated rain event and compared with the indigenous E. coli population. The concentration of E. coli O157:H7 in the leachates followed a similar trend to that of nonpathogenic E. coli. Significantly greater densities of generic and pathogenic E. coli were recovered in the leachates generated from sheep faeces compared with cattle faeces. Pathogen metabolic activity was also significantly greater in sheep faeces. Our findings show that E. coli O157:H7 may readily leach from ruminant faeces during rain events. The bacterium leaches more freely from sheep faeces than from cattle faeces and displays greater metabolic activity within sheep leachate. A novel inoculation technique was developed that allowed the determination of both popula...Continue Reading

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Citations

Apr 5, 2012·The Journal of Dairy Research·Nawfal A AlhelfiA Prysor Williams
Dec 2, 2010·Foodborne Pathogens and Disease·Witold A Ferens, Carolyn J Hovde
Sep 26, 2009·Journal of Applied Microbiology·B FremauxC Rozand
Feb 13, 2014·Letters in Applied Microbiology·E M Moriarty, B J Gilpin
Aug 22, 2008·FEMS Microbiology Letters·A Prysor WilliamsDavid L Jones
Aug 13, 2013·The Veterinary Record·N A AlhelfiA P Williams
Apr 6, 2019·Journal of Environmental Quality·Paul O'CallaghanDaire Ó hUallacháin

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