Lead acetate toxicity in vitro: Dependence on the cell composition of the cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M-G ZurichP Honegger

Abstract

It is well known that exposure to low doses of lead causes long-lasting neurobehavioural deficits, but the cellular changes underlying these behavioural changes remain to be elucidated. A protective role of glial cells on neurons through lead sequestration by astrocytes has been proposed. The possible modulation of lead neurotoxicity by neuron-glia interactions was examined in three-dimensional cultures of foetal rat telencephalon. Mixed-brain cell cultures or cultures enriched in either neurons or glial cells were treated for 10 days with lead acetate (10(-6) m), a concentration below the limit of cytotoxicity. Intracellular lead content and cell type-specific enzyme activities were determined. It was found that in enriched cultures neurons stored more lead than glial cells, and each cell type alone stored more lead than in co-culture. Moreover, glial cells but not neurons were more affected by lead in enriched culture than in co-culture. These results show that neuron-glia interactions attenuate the cellular lead uptake and the glial susceptibility to lead, but they do not support the idea of a protective role of astrocytes.

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Citations

Oct 25, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M M Garber, A S Heiman
Aug 11, 2006·Reviews on Environmental Health·Florianne Monnet-TschudiPaul Honegger
Sep 19, 2002·Journal of Neuroscience Research·Marie-Gabrielle ZurichFlorianne Monnet-Tschudi
Jun 30, 2009·Brain Research Bulletin·M G Zurich, F Monnet-Tschudi
Dec 18, 2007·Toxicology and Applied Pharmacology·Florianne Monnet-TschudiPaul Honegger
Mar 22, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S VichiM G Zurich
Dec 16, 2011·Environmental Toxicology and Chemistry·Dong-Ha NamNiladri Basu
Nov 16, 2004·Toxicology and Applied Pharmacology·M-G ZurichF Monnet-Tschudi
Sep 8, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marie-Gabrielle ZurichPaul Honegger

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