Lead Affects Vitamin D Metabolism in Rats

Nutrients
A RahmanK M Khan

Abstract

A negative association between blood lead and vitamin D concentrations has been reported, however, experimental data on the effect of lead (Pb) on vitamin D metabolism is scarce. We investigated the effects of Pb on serum vitamin D metabolites, vitamin D activating enzymes and vitamin D receptor (VDR) in rats. Newborn Wistar rat pups were exposed to 0.2% Pb-acetate via their dams' drinking water from post-natal day (PND) 1 to 21 and directly in drinking water until PND30. Serum 25-hydroxyvitamin D was analyzed with LC-MS/MS and 1,25-dihydroxyvitamin D with an immunoassay. Tissue expression of vitamin D activating enzymes and VDR were measured by Western blot and immunohistochemistry. Serum 25-hydroxyvitamin D was significantly decreased at both PND21 and PND30, whereas 1,25-dihydroxyvitamin D was decreased (p< 0.05) only at PND21 in the Pb-exposed rats. Expression of renal 1-α-hydroxylase was decreased by Pb only at PND21 (p< 0.05) but the brain 1-α-hydroxylase was not affected. Hepatic 25-hydroxylase expression was significantly decreased at PND21 but significantly increased at PND30 by Pb exposure. VDR expression in the brain was increased at both PND21 and PND30 (p< 0.05). These results suggest that Pb interferes with vitami...Continue Reading

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Citations

Jun 13, 2019·Medicina·Egidijus ŠimoliūnasVirginija Bukelskienė
Apr 24, 2020·Orthopedics·Amy L MeyersRyan A Nelson
Jan 6, 2021·Neurotoxicity Research·Hossein HosseiniradNousha Afsharzadeh
Jul 4, 2021·Environmental Science and Pollution Research International·Harith Abdulrhman AhmedThulfiqar Fawwaz Mutar

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

SPSS
Gene Tools Image Analysis
Thermo Scientific iTEVA
Chromsystems
Syngene gene tools

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