Lead treatment in vitro at early developmental stage of bone marrow-derived macrophages enhances NO production through IL-1beta and IL-6 but not TNF-alpha

Toxicology
J S SongS D Yang

Abstract

Lead (Pb(2+)) is known to decrease or block nitric oxide (NO) production by mature macrophages (mphi). Bone marrow cells were treated with various doses of lead in vitro and the morphological and functional changes were observed. Bone marrow cells were treated with various doses of lead (1, 10, 20 and 50 microM) at the start of culture with mphi growth factor (CSF-1), and after 6-7 days of culture, the resultant mphi (bone marrow-derived mphi, BMDM) showed decreased NO production. Unexpectedly, BMDM from the lowest does of lead treatment (1.0 microM) showed increased NO production. The increased NO production was due to increased expression of the iNOS gene and concurrent enhanced transcript levels of proinflammatory cytokines such as IL-1beta and IL-6, but not TNF-alpha. Lead treatment on mature BMDM showed decreased NO production in a dose-dependent manner. These results suggest that a low dose of lead affects developmental characteristics of BMDM through different proinflammatory cytokines, and the lead effects on precursor cells of mphi and mature mphi are different.

References

Mar 1, 1992·Journal of Pediatric Health Care : Official Publication of National Association of Pediatric Nurse Associates & Practitioners·K E Mitchell, C Hobbie
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A L Witsell, L B Schook
Nov 30, 1988·Biochemical and Biophysical Research Communications·J B HibbsE M Rachlin
Jul 1, 1995·Molecular Immunology·S D YangM S Rutherford
Nov 1, 1994·Trends in Endocrinology and Metabolism : TEM·J D LaskinD L Laskin

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Citations

May 22, 2007·Toxicology and Applied Pharmacology·Donghong GaoDavid A Lawrence
May 28, 2014·Journal of Biochemical and Molecular Toxicology·Jane Kasten-Jolly, David A Lawrence
Sep 22, 2007·Human & Experimental Toxicology·M ValentinoL Soleo
Nov 5, 2019·Toxicology Mechanisms and Methods·Ibraheem M AttafiHesham M Korashy
Sep 13, 2020·Biological Trace Element Research·Raghumoy GhoshPraveen Sharma
Aug 8, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mary L S QueirozChrislaine O Soares

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