Leading strand specific spontaneous mutation corrects a quasipalindrome by an intermolecular strand switch mechanism

Journal of Molecular Biology
W A RoscheR R Sinden

Abstract

Imperfect inverted repeats or quasipalindromes can undergo spontaneous, often complex mutational events that correct them to perfect palindromes. Two models that depend on the quasipalindrome providing a template for a specific mutational event have been described to explain this mutation: an intramolecular and an intermolecular strand switch model. A 17bp quasipalindrome containing a -1 deletion within the chloramphenicol acetyl transferase (CAT) gene in plasmid pJT7 undergoes a spontaneous +1 frameshift mutation that creates a perfect inverted repeat and a Cm(r) phenotype. By analyzing this mutation frequency in two plasmids that contain the CAT gene in either orientation with respect to the origin of replication, we show that the specific frameshift occurs preferentially in the leading strand during DNA replication. Due to the availability and proximity of the lagging strand template as a single strand during replication of the quasipalindrome in the leading but not lagging strand, we suggest that the specificity for the leading strand correction is due to a leading strand specific intermolecular strand switch rather than an intramolecular strand switch. To test this hypothesis, we have designed a genetic selection to detect...Continue Reading

References

Aug 1, 1992·PCR Methods and Applications·R S ChaW G Thilly
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B O Parker, M G Marinus
Aug 1, 1991·Human Genetics·D N Cooper, M Krawczak
Sep 4, 1990·Biochemistry·T A Kunkel
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A OhshimaM Inouye
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N BaranH Manor
Jan 1, 1990·Annual Review of Genetics·L S Ripley
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R A GibbsC T Caskey
Jan 1, 1988·Annual Review of Biochemistry·C S McHenry
Dec 25, 1984·Journal of Molecular Biology·D T Weaver, M L DePamphilis
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B W Glickman, L S Ripley
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L S Ripley
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R S ChaH Zarbl
Nov 1, 1943·Genetics·S E Luria, M Delbrück

❮ Previous
Next ❯

Citations

Oct 27, 1998·Nature Biotechnology·L H Caporale
Jul 25, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M M SlupskaJ H Miller
Sep 21, 2013·Nucleic Acids Research·Matthew R NorthamPolina V Shcherbakova
Jul 23, 1999·Annals of the New York Academy of Sciences·L H Caporale
Jul 23, 1999·Annals of the New York Academy of Sciences·R R SindenW A Rosche
Oct 18, 2005·Biochemical and Biophysical Research Communications·Allison M Faucett, Angel L Islas
Aug 4, 2005·Journal of Biomedical Science·Yi-Kuang ChuangWoei-horng Fang
Aug 13, 2008·Journal of Molecular Evolution·Emmanuel D Ladoukakis, Adam Eyre-Walker
Mar 5, 2005·Molecular Biology and Evolution·Seanna J McTaggart, Teresa J Crease
Apr 3, 2018·Genome Biology and Evolution·Bar LaviEinat Hazkani-Covo
Oct 22, 2020·Microorganisms·Virali J ParekhRichard R Sinden
Sep 15, 2000·Journal of Molecular Biology·M ViswanathanS T Lovett
May 9, 2002·Mutation Research·Vera I HashemRichard R Sinden
Jul 10, 2003·Trends in Genetics : TIG·Vera van NoortRichard R Sinden
Dec 27, 2005·Journal of Molecular Biology·Bethany E Dutra, Susan T Lovett
Apr 1, 2006·Journal of Molecular Biology·Gary E SchultzJohn W Drake
Jun 30, 2006·DNA Repair·Susanna M Lewis, Atina G Coté
Nov 21, 2021·Scientific Reports·May Abraham, Einat Hazkani-Covo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.