Mar 30, 2020

Subtilase activity in the intrusive cells mediates haustorium maturation in parasitic plants

BioRxiv : the Preprint Server for Biology
Abhinav GanesanKen Shirasu

Abstract

Parasitic plants that infect crops are devastating to agriculture throughout the world. They develop a unique inducible organ called the haustorium, which connects the vascular systems of the parasite and host to establish a flow of water and nutrients. Upon contact with the host, the haustorial epidermal cells at the interface with the host differentiate into specific cells called intrusive cells that grow endophytically towards the host vasculature. Then, some of the intrusive cells re-differentiate to form a xylem bridge that connects the vasculatures of the parasite and host. Despite the prominent role of intrusive cells in host infection, the molecular mechanisms mediating parasitism in the intrusive cells are unknown. In this study, we investigated differential gene expression in the intrusive cells of the facultative parasite Phtheirospermum japonicum in the family Orobanchaceae by RNA-Sequencing of laser-microdissected haustoria. We then used promoter analyses to identify genes that are specifically induced in intrusive cells, and used promoter fusions with genes encoding fluorescent proteins to develop intrusive cell-specific markers. Four of the intrusive cell-specific genes encode subtilisin-like serine proteases (SB...Continue Reading

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Mentioned in this Paper

Bounded by
Pathogenic Organism
Inhibitors
Plumbum metallicum, homeopathic preparation
Pharmacologic Substance
Adaptation
Chemicals
Lead compound
Learning
Lead Measurement

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