Lectin--peroxidase conjugates in histopathology of gastrointestinal mucosa

Virchows Archiv. A, Pathological Anatomy and Histopathology
W D KuhlmannK Wurster

Abstract

Peroxidase (HRP) conjugates of Dolichos biflorus agglutinin (DBA), Arachis hypogaea agglutinin (PNA) and Ulex europaeus agglutinin I (UEA I) were used as specific molecular probes in order to describe histological patterns of carbohydrate moieties in glycoconjugates of gastric mucosa. Specimens of normal mucosa, metaplasias and carcinomas were fixed, embedded in paraffin and stained with lectins by a postembedding technique. In normal mucosa, DBA reacted with surface epithelial, parietal and antral gland cells; neck cells did not react. In single goblet cell metaplasia, with few exceptions, goblet cells showed DBA binding. In contrast, mainly negative goblet cells were seen in intestinal metaplasia. In gastric carcinomas of intestinal or diffuse types, heterogeneous staining patterns were observed. Large numbers of mucus-producing surface epithelial, neck and antral gland cells stained with PNA-HRP conjugates. Parietal cells did not react. Among the chief cells only few showed PNA binding. In single goblet cell metaplasia, equal numbers of goblet cells were positive or negative, whereas goblet cells in intestinal metaplasia were mainly unstained. Carcinomas contained both PNA-positive and PNA-negative cells; PNA-positive cells ...Continue Reading

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Citations

Jan 1, 1988·Histochemistry·G BarresiG Magazzù
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·P MöllerK Schwechheimer
Jan 1, 1983·Virchows Archiv. A, Pathological Anatomy and Histopathology·K WursterW D Kuhlmann
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May 25, 2021·Frontiers in Molecular Biosciences·Rita MatosCelso A Reis

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