Lectin affinity electrophoresis of alpha-fetoprotein in cancer diagnosis.

Electrophoresis
K Taketa, H Hirai

Abstract

Lectin affinity electrophoresis of serum alpha-fetoprotein (AFP) was carried out on samples obtained from patients with benign and malignant diseases and on cord blood, and separated AFP bands were detected by antibody-affinity blotting. The following major bands were identified by determination of kinetic constants: AFP-C1 and -C2 with concanavalin A, AFP-L1, -L2 and -L3 with Lens culinaris agglutinin A, AFP-P1, -P2, -P3, -P4 and -P5 with erythroagglutinating phytohemagglutinin, and AFP-A1, -A2 and -A3 with Allomyrina dichotoma lectin. AFP bands with the lowest number had either low or no affinity and those with higher numbers had higher affinities for respective lectins. AFP from cord blood and chronic liver disease was characterized by the predominance of AFP-C2, AFP-L1, AFP-P2 and AFP-A3. Hepatocellular carcinoma was differentiated from the benign liver disease by increased proportions of AFP-L3 and AFP-P4. Extrahepatic tumors had additional increases of AFP-C1, AFP-L2, AFP-P5 and AFP-A1 (or slow-migrating AFP-Als, particularly in yolk sac tumor).

References

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Citations

Jun 1, 1991·Electrophoresis·D SchranzG I Abelev
Jan 31, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·K ShimizuK Taketa
Mar 23, 2011·Journal of Gastroenterology and Hepatology·Yutaka AoyagiTakeshi Suda
Dec 1, 1994·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·W L van NoortH G van Eijk
Oct 1, 1995·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·I KatnikW Dobryszycka
Jun 10, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Evi N Debruyne, Joris R Delanghe
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Jan 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H OkaS Yamamoto
Jan 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y AoyagiH Asakura
Dec 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Taketa
Aug 9, 2012·The Journal of Biological Chemistry·Yuka KobayashiHirokazu Kawagishi
May 16, 2008·Journal of Proteome Research·Takatoshi NakagawaAkihiro Kondo
Sep 13, 1991·Journal of Chromatography·K Taketa

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