PMID: 3755163Apr 1, 1986Paper

Lectin and antibody labelling of developing rat photoreceptor cells: an electron microscope immunocytochemical study

Journal of Neurocytology
D Hicks, C J Barnstable

Abstract

Lectin and rhodopsin antibody binding sites were studied in developing and adult rat photoreceptors in order to compare changes in the total carbohydrate pool with the movement of a known glycoprotein rhodopsin. Electron microscope immunocytochemical techniques utilizing modified colloidal gold methods were used. At birth, all three lectins - Concanavalin A (ConA), Ricinus communis agglutinin II (RCA II) and wheat germ agglutinin (WGA) - showed heavy labelling of the photoreceptor surface scleral to the outer limiting membrane. At the same age, a monoclonal antibody against rhodopsin, RET-P1, revealed sparse labelling of only occasional immature photoreceptor surfaces. At postnatal day 4(P4), all three lectins showed variable binding to the inner segment and along the length of the newly forming connecting cilium. There was generally a region of more intense label at the base of the cilium. RET-P1 binding to P4 retina showed a discontinuous distribution, with heavily labelled inner segments being adjacent to unlabelled inner segments. This pattern indicates that the initial expression of rhodopsin is not a coordinate event but occurs in discrete cells, possibly related to the end of mitosis. RET-P1 binding at this age was reduc...Continue Reading

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Citations

Jun 1, 1993·Journal of Neurocytology·L AguilarL J Robles
Jan 1, 1987·Molecular Neurobiology·C J Barnstable
Mar 17, 2009·Fish Physiology and Biochemistry·R MatsuuraY Ishibashi
Jan 1, 1988·Vision Research·V P GaurP V Sarthy
Jan 1, 1988·Neuroscience Research. Supplement : the Official Journal of the Japan Neuroscience Society·C J BarnstableJ E Treisman
Feb 1, 1990·The European Journal of Neuroscience·Kevin M. McGrail, Kathleen J. Sweadner
Jun 28, 2005·Investigative Ophthalmology & Visual Science·Joseph A BrzezinskiTom Glaser
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Oct 1, 1991·Experimental Eye Research·S B SmithP J O'Brien
Jul 13, 2000·Cell Motility and the Cytoskeleton·U Wolfrum, A Schmitt
Feb 5, 2019·Molecular Neurobiology·Amy Q Lu, Colin J Barnstable
Apr 13, 2019·Journal of Cell Science·Abhishek ChadhaDavid S Williams
Apr 1, 1988·Molecular and Cellular Biology·J E TreismanC J Barnstable
Sep 1, 1989·Experimental Eye Research·I NirD S Papermaster

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