Lectin-based analysis of fucosylated glycoproteins of human skim milk during 47 days of lactation

Glycoconjugate Journal
Jolanta Lis-KuberkaMagdalena Orczyk-Pawiłowicz

Abstract

Glycoproteins of human milk are multifunctional molecules, and their fucosylated variants are potentially active molecules in immunological events ensuring breastfed infants optimal development and protection against infection diseases. The expression of fucosylated glycotopes may correspond to milk maturation stages. The relative amounts of fucosylated glycotopes of human skim milk glycoproteins over the course of lactation from the 2(nd) day to the 47(th) day were analyzed in colostrums, transitional and mature milk samples of 43 healthy mothers by lectin-blotting using α1-2-, α1-6-, and α1-3-fucose specific biotinylated Ulex europaeus (UEA), Lens culinaris (LCA), and Lotus tetragonolobus (LTA) lectins, respectively. The reactivities of UEA and LCA with the milk glycoproteins showed the highest expression of α1-2- and α1-6-fucosylated glycotopes on colostrum glycoproteins. The level of UEA-reactive glycoproteins from the beginning of lactation to the 14(th) day was high and relatively stable in contrast to LCA-reactive glycoproteins, the level of which significantly decreased from 2-3 to 7-8 days then remained almost unchanged until the 12(th)-14(th) days. Next, during the progression of lactation the reactivities with both l...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P FalkS Normark
Jan 29, 1999·Physiological Reviews·D Vestweber, J E Blanks
Jun 14, 2000·Archives of Biochemistry and Biophysics·E LandbergP Påhlsson
Nov 25, 2000·Journal of Molecular Biology·G F AudetteL T Delbaere
Jan 1, 2003·The Journal of Infectious Diseases·Henry F GomezThomas G Cleary
Mar 15, 2003·The Journal of Biological Chemistry·Louise RoylePauline M Rudd
Mar 26, 2003·Glycobiology·Daniel J Becker, John B Lowe
Oct 23, 2004·The Journal of Infectious Diseases·Xi JiangLarry K Pickering
Sep 1, 2005·Glycoconjugate Journal·Anki GustafssonJan Holgersson
Oct 22, 2005·The Journal of Clinical Investigation·Marloes A NaardingWilliam A Paxton
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Heather E MurreyLinda C Hsieh-Wilson
Mar 18, 2006·The Journal of Biological Chemistry·Clémentine PerrierBlaise Corthésy
Apr 6, 2006·Glycobiology·Simon ParryAnn Harris
Sep 16, 2006·Glycobiology·Bing MaDiane E Taylor
Nov 23, 2006·Microbiology and Immunology·Heidi Christina BesslerLoreny Gimenes Giugliano
Mar 28, 2008·Glycoconjugate Journal·Albert M WuZhangung Yang
Jun 9, 2009·Journal of Pediatric Gastroenterology and Nutrition·UNKNOWN ESPGHAN Committee on NutritionJohannes van Goudoever
Jul 7, 2009·Annual Review of Nutrition·Bing Wang
Feb 13, 2010·The Journal of Pediatrics·Allan Walker
Apr 27, 2010·Journal of Agricultural and Food Chemistry·John W FroehlichCarlito B Lebrilla
Dec 14, 2012·Gut Microbes·Elizabeth A MagaJames D Murray
Dec 21, 2012·Chemical Society Reviews·Anna BernardiAnne Imberty
May 24, 2013·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Bo Liu, David S Newburg
Aug 14, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Iglika Djoumerska-AlexievaAndrey Tchorbanov
Sep 10, 2013·Biochemistry. Biokhimii︠a︡·D S Newburg
Feb 7, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sara F RinaldiJane E Norman
Jun 4, 2014·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Magdalena Orczyk-PawiłowiczIwona M Kątnik-Prastowska
Dec 3, 2014·Early Human Development·Marita BroadhurstThomas Wheeler
Dec 3, 2014·Clinical Biochemistry·Magdalena Orczyk-PawiłowiczIwona Kątnik-Prastowska
Jan 30, 2015·Journal of Proteome Research·Jincui HuangCarlito B Lebrilla
Mar 31, 2015·Frontiers in Immunology·Nathalie KoningYvette Van Kooyk

❮ Previous
Next ❯

Citations

Apr 23, 2020·Nutrients·Magdalena Orczyk-Pawiłowicz, Jolanta Lis-Kuberka
Oct 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jolanta Lis-KuberkaMagdalena Orczyk-Pawiłowicz
Aug 28, 2020·Frontiers in Pediatrics·Matylda Czosnykowska-ŁukackaMagdalena Orczyk-Pawiłowicz
Jan 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Susana Figueroa-Lozano, Paul de Vos
Jun 5, 2020·Journal of Agricultural and Food Chemistry·Mohèb ElwakielHenk A Schols

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
Protein Assay
electrophoresis

Software Mentioned

myImageAnalysis
STATISTICA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine
Magdalena Orczyk-PawiłowiczIwona Kątnik-Prastowska
Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine
Magdalena Orczyk-PawiłowiczIwona Kątnik-Prastowska
© 2021 Meta ULC. All rights reserved