Lectin-binding and dissociation/reconstitution studies on the trichocysts of the dinoflagellate Oxyrrhis marina

Protoplasma
Erhard RhielRalf Rabus

Abstract

Trichocyst-enriched fractions were isolated from the dinoflagellate Oxyrrhis marina and subjected to protein staining and lectin-binding studies, to dissociation experiments using heat, and to solubilization/reconstitution experiments using 6 M guanidine hydrochloride. The trichocysts could be stained by Alcian Blue and became labeled by the lectin Concanavalin A, but not by wheat germ agglutinin. The trichocysts did not dissociate when the fractions were heated for 5 min at 40 or 50 °C. Heating at 60 °C resulted in the dissociation of trichocysts into irregular filamentous structures. These filaments were still present when the fractions were incubated for 5 min at temperatures of 70 and 80 °C. Reassembly was not achieved by subsequent cooling steps. The disintegration of trichocysts was also achieved in 6 M guanidine hydrochloride, and reassembly into filamentous structures, similar to those obtained by heat, occurred after dialysis against distilled water. Electron microscopy revealed that the filaments created either by heat or using guanidine hydrochloride by far did not resemble native trichocysts. They were much thinner (5-7 nm in width), missed the characteristic striation of electron-dense and -transparent lines along ...Continue Reading

References

Jan 10, 1992·Biochimica Et Biophysica Acta·R Glas-AlbrechtH Plattner
Apr 1, 1962·Canadian Journal of Microbiology·R R GUILLARD, J H RYTHER
Jan 22, 2004·The Journal of Eukaryotic Microbiology·Giovanna Rosati, Letizia Modeo
Dec 7, 2006·FEMS Microbiology Letters·Emily C RobertsEmma C Wootton
Jan 18, 2007·Environmental Microbiology·Emma C WoottonEmily C Roberts
Oct 19, 1990·European Journal of Protistology·R Glas-AlbrechtH Plattner
Jun 3, 2014·European Journal of Protistology·Silke AmmermannErhard Rhiel
Jun 29, 2016·Protoplasma·Erhard Rhiel

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Citations

Apr 16, 2019·Plant Signaling & Behavior·Hyeran Kim, Jongseok Lim

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Methods Mentioned

BETA
transmission electron microscopy
electrophoresis
light microscopy
confocal microscopy
electron microscopy

Software Mentioned

Image SP
Mascot
DELTA
BLAST
ProteinScape

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