Lectin binding to cell surfaces: comparisons between normal and migrating corneal epithelium

Developmental Biology
I K GipsonS J Spurr

Abstract

Comparisons were made between cell surfaces of normal and migrating corneal epithelium of the rat by localizing and/or quantifying concanavalin A (Con A) and wheat germ agglutinin (WGA) binding. Our results indicate that apical cell surfaces of the leading edge of a migrating sheet of epithelium differ from those of normal epithelium and that the various cell layers within the stratified normal epithelium have different lectin-binding characteristics. Three methods of monitoring lectin binding to cell surfaces were employed. Based on ferritin-conjugated Con A, ferritin-conjugated WGA, and [3H]Con A binding, apical cell membranes of migrating epithelia bind more Con A and WGA than do apical membranes of superficial cells of normal stratified epithelia. With both fluorescein isothiocyanate (FITC)-Con A and -WGA, membranes of all the cells of the leading edge of the migrating sheet fluoresce intensely. FITC-Con A binding of normal stratified epithelium is relatively uniform through all cell layers with no discernible staining of the apical membrane of superficial cells. With FITC-WGA, however, fluorescence is present only on basal cell layers but not on superficial cells. These data demonstrate that apical cell surface sugars on a...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J F Ash, S J Singer
Jan 4, 1974·Nature·R D BerlinH H Yin
Jul 1, 1982·Developmental Biology·I K Gipson, T C Kiorpes

❮ Previous
Next ❯

Citations

Feb 16, 2000·Progress in Retinal and Eye Research·J M WolosinA Tieng
Jan 1, 1991·The British Journal of Ophthalmology·P N BishopR W Stoddart
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·S de BentzmannE Puchelle
Jun 1, 1991·The Journal of Clinical Investigation·M C PlotkowskiE Puchelle
Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·S PhilipponK Morgenroth
Jan 1, 1993·Progress in Histochemistry and Cytochemistry·L D Hazlett
May 3, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B J Patchell, D R Dorscheid
Aug 1, 1984·The Journal of Experimental Zoology·D J Donaldson, J T Mahan
Oct 1, 1994·The Histochemical Journal·A TuoriH Uusitalo
Jun 6, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sima AllahverdianDelbert R Dorscheid
Aug 27, 2013·Current Opinion in Allergy and Clinical Immunology·Flavio MantelliPablo Argüeso
Aug 21, 2015·Medicinal Research Reviews·Xiuli DanTzi Bun Ng
Jan 1, 1992·Current Eye Research·M D McCartney, D Cantu-Crouch
Sep 17, 1999·Current Eye Research·A J SweattR M Davis
Apr 1, 1987·Current Eye Research·M D McCartneyB J McLaughlin
Oct 1, 1985·The Journal of Investigative Dermatology·A Takashima, F Grinnell
Dec 13, 2018·Journal of Ophthalmology·Islam Mahmoud Hamdi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Barrett Esophagus

Barrett’s esophagus if a serious complication of gastroesophageal reflux disease during which the normal esophageal lining changes to tissue that resembles intestinal lining. Here is the latest research.