Lectin-carbohydrate affinity measured using a quartz crystal microbalance

Journal of Colloid and Interface Science
Kateryna LebedMałgorzata Lekka

Abstract

The association of two molecules is described by two parameters, association equilibrium and association rate constants, which are characteristic for a given type of interaction. Usually, they are determined for interacting molecules dissolved in solution. However, for many applications one type of molecules is immobilized on a substrate, which may influence the binding kinetics. The studied complex of concanavalin A and carboxypeptidase Y belongs to the lectin-carbohydrate type of interaction involving the recognition of oligosaccharide moieties. The concanavalin A was immobilized on a gold electrode of quartz crystal, while carboxypeptidase Y was added to a buffer (Tris-buffered saline). The constants describing the association of the investigated molecules were determined on the basis of measurements performed using a quartz crystal microbalance in liquid. The obtained values were (0.59+/-0.01)x10(6) M(-1) for the association equilibrium constant and (5.6+/-0.1)x10(4) M(-1)s(-1) for the association rate constant. The saturation binding experiment gave another value of the association constant, (2.7+/-0.02)x10(6) M(-1). The comparison of obtained values with previously published ones verifies that the molecule orientation and...Continue Reading

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Citations

May 14, 2011·Analytica Chimica Acta·Maria E YakovlevaBengt Danielsson
Dec 22, 2015·Carbohydrate Polymers·Chuntao ChenDongping Sun
Aug 4, 2011·Journal of Molecular Recognition : JMR·Bernd Becker, Matthew A Cooper
Mar 18, 2009·Medicinal Research Reviews·Shan JinBinghe Wang
Apr 29, 2014·Biosensors & Bioelectronics·Fohona S Coulibaly, Bi-Botti C Youan
Oct 22, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Adriano SantosPaulo R Bueno
Jan 10, 2018·Critical Reviews in Analytical Chemistry·O D Hendrickson, A V Zherdev
Aug 19, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Joanna ZemłaJoanna Raczkowska
Sep 11, 2010·Analytical Chemistry·Sabine SzuneritsAloysius Siriwardena

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