Lectin-carbohydrate recognition mechanism of Plasmodium berghei in the midgut of malaria vector Anopheles stephensi using quantum dot as a new approach

Acta Tropica
Hamid Reza BasseriMohammad R Abai

Abstract

Potential targets of Plasmodium ookinetes at the mosquito midgut walls were investigated in relation to interfering malarial transmission. In this study, the essential application of Quantum Dots (QDs) was used to examine the interaction between Plasmodium berghei ookinetes and the Anopheles stephensi midgut, based on lectin-carbohydrate recognition. Two significant lectins were utilized to determine this interaction. Two QDs, cadmium telluride (CdTe)/CdS and cadmium selenide (CdSe)/CdS, were employed in staining Plasmodium ookinete to study its interaction in the midgut of the mosquito vector in vivo. Concurrently, two lectins, wheat germ agglutinin (WGA) and concanavalin A (Con A), were inadvertently exploited to mask lectin binding sites between ookinetes and mosquito midgut cells. The numbers of ookinetes in both lumen and epithelial cells were eventually counted, following adequate preparation of wax sections extracted from whole midgut, and subsequent examination using a differential interference contrast a fluorescence microscopic technique. Interestingly, we detected that neither of the QDs mutated ookinete invasion into the midgut cells of the investigated mosquitoes. QD staining of ookinetes remained permanent despite...Continue Reading

References

Feb 1, 1988·The Journal of protozoology·M F LimaF Kierszenbaum
Feb 1, 1994·International Journal for Parasitology·P F Billingsley
Feb 13, 2001·Insect Biochemistry and Molecular Biology·M Shahabuddin, A Costero
Feb 28, 2002·Trends in Microbiology·Isabel Roditi, Matthias Liniger
Oct 2, 2003·Nature Biotechnology·George M Whitesides
Oct 3, 2003·Biochemical and Biophysical Research Communications·Evelize FollyGeorgia C Atella
Jan 9, 2004·Applied and Environmental Microbiology·Liang ZhuWen-Tso Liu
Jul 22, 2004·European Journal of Biochemistry·Juan L Jurat-Fuentes, Michael J Adang
Oct 7, 2004·Applied and Environmental Microbiology·Lai Yoke LeeShih Wei Wong
Aug 28, 2007·International Journal of Nanomedicine·Norah O'FarrellBenjamin R Horrocks
Sep 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rhoel R DinglasanMarcelo Jacobs-Lorena
Aug 6, 2008·Trends in Parasitology·Peter F BillingsleyJason L Rasgon
Jun 25, 2009·Journal of the American Chemical Society·Brian J WalkerMoungi G Bawendi
Apr 1, 2010·Nature Methods·Fabien PinaudMaxime Dahan
Apr 15, 2010·Journal of Nanobiotechnology·Hideyuki TerazonoKenji Yasuda
Apr 20, 2010·Angewandte Chemie·David A GiljohannChad A Mirkin
Oct 12, 2010·Journal of the American Chemical Society·Changfeng WuDaniel T Chiu
Feb 2, 2011·ACS Nano·Yuhui JinDaniel T Chiu
Mar 23, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Meng WangShukun Xu
May 4, 2011·Memórias do Instituto Oswaldo Cruz·Cecilia Stahl VieiraDenise Feder
May 11, 2011·Malaria Journal·Min-Je KuLucio H Freitas-Junior
Oct 11, 2011·Advanced Drug Delivery Reviews·Hedi MattoussiHyon Bin Na
Mar 13, 2012·International Journal for Parasitology·Fiona AngrisanoJake Baum
Apr 21, 2012·Journal of the American Chemical Society·Yang-Hsiang ChanDaniel T Chiu
Jul 18, 2012·The American Journal of Tropical Medicine and Hygiene·Kailash P Patra, Joseph M Vinetz
Aug 10, 2013·Journal of Biomedical Nanotechnology·Hisatoshi KobayashiTsuyoshi Takato

❮ Previous
Next ❯

Citations

Aug 3, 2021·Frontiers in Microbiology·Felix GoerdelerOren Moscovitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.