Lectin from Canavalia brasiliensis seeds (ConBr) is a valuable biotechnological tool to stimulate the growth of Rhizobium tropici in vitro.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Mayron Alves de VasconcelosRicardo Pires dos Santos

Abstract

To study the interactions between a Rhizobium tropici strain and lectins isolated from the seeds of Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr), a lectin fluorescence assay was performed. In addition, an experiment was designed to evaluate the effect of the two lectins on bacterial growth. Both lectins were found to bind to R. tropici cells, but the interactions were inhibited by D-mannose. Interestingly, only ConBr stimulated bacterial growth in proportion to the concentrations used (15.6-500 μg/mL), and the bacterial growth stimulation was inhibited by D-mannose as well. Structure/Function analyses by bioinformatics were carried out to evaluate the volume and carbohydrate recognition domain (CRD) configuration of ConA and ConBr. The difference of spatial arrangement and volume of CRD may indicate the variation between biological activities of both lectins. The results suggest that ConBr could be a promising tool for studies focusing on the interactions between rhizobia and host plants.

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Citations

Jan 1, 2014·BioMed Research International·Francisco Vassiliepe Sousa ArrudaAlexandre Holanda Sampaio
Jul 2, 2014·BioMed Research International·Mayron Alves VasconcelosMaria Olivia Pereira
May 21, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mayron Alves de VasconcelosEdson Holanda Teixeira
Jan 5, 2019·Current Protein & Peptide Science·Benildo Sousa CavadaKyria Santiago Nascimento
Sep 18, 2018·Archives of Biochemistry and Biophysics·Mayron Alves de VasconcelosAndré Luis Coelho da Silva

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