Lectin-histochemistry: glycogenosis in cattle

Veterinary Research Communications
P ZlotowskiDavid Driemeier

Abstract

Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.

References

Jan 1, 1978·Advances in Carbohydrate Chemistry and Biochemistry·I J Goldstein, C E Hayes
Jan 1, 1977·Australian Veterinary Journal·R D Jolly, W J Hartley
Jan 1, 1975·Journal of Comparative Pathology·B W Manktelow, W J Hartley
Nov 1, 1986·The Histochemical Journal·R A HennigarS S Spicer
Sep 1, 1987·Australian Veterinary Journal·P J HealyK G Reichmann
Jan 1, 1969·Acta Neuropathologica·B SandströmP A Ockerman
Mar 1, 1970·The Journal of Small Animal Practice·M Ajmal, A H Hayward
Mar 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M B Hancock
Dec 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J AlroyM E Pereira
May 1, 1981·Australian Veterinary Journal·B M O'SullivanC A Sewell
Mar 1, 1982·Neuropathology and Applied Neurobiology·R D CookR B Richards
Aug 1, 1981·The Journal of Pathology·J M HowellJ M Gawthorne
Nov 1, 1993·Australian Veterinary Journal·K G ReichmannE J Thistlethwaite
Feb 15, 1993·Biochemical and Biophysical Research Communications·H A WisselaarR D Drinkwater
Mar 21, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J A DennisP J Healy
Jun 21, 2002·Australian Veterinary Journal·J A DennisK G Reichmann
Apr 17, 2004·Journal of Neurochemistry·Angus M Brown

❮ Previous
Next ❯

Citations

Mar 31, 2009·International Journal of Molecular Sciences·Maria Teresa MontagnaGiovanna Barbuti
May 2, 2017·Genes & Genomics·Aleksandra CiepłochEwa Poławska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.