Lectin staining of the uterovaginal junction and sperm-storage tubule epithelia in broiler hens

Poultry Science
M R Bakst, Gary Bauchan

Abstract

Mammalian sperm bind to terminal carbohydrates associated with glycoconjugates on the apical surface of oviduct epithelial cells in the caudal region of the oviduct and undergo cellular and molecular modifications associated with capacitation prior to ovulation. In contrast, chicken sperm are stored for up to 23 d in sperm-storage tubules (SST) localized in the uterovaginal junction (UVJ). Little is known of the cellular and molecular mechanisms that regulate sperm storage in and release from the SST. The purpose of this study was to identify glycoconjugates associated with the SST epithelial cell surface using lectins. Virgin hens and hens of higher and lower fertility in egg production for 6 to 16 wk were used in this study. Sections of UVJ mucosa containing SST were stained with fluorescent conjugated lectins and examined by confocal microscopy. Carbohydrate moieties associated with the UVJ and SST epithelia differed in their lectin binding patterns. No differences in the lectin binding patterns within the 2 epithelia were discernible between the virgin and younger and older hens. Minor differences were observed between the higher and lower fertility hens. Only lectins specific for galactose and N-acetylgalactosamine moietie...Continue Reading

References

Sep 1, 1973·Journal of Reproduction and Fertility·M D Tingari, P E Lake
Jan 1, 1983·British Poultry Science·K Ashizawa, H Nishiyama
Jan 1, 1993·Reproduction, Fertility, and Development·M R Bakst
Jul 24, 2008·The International Journal of Developmental Biology·Susan S Suarez
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Aug 18, 2009·Theriogenology·R Talevi, R Gualtieri
Aug 18, 2009·Animal Reproduction Science·Silvana A ApichelaDora C Miceli

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Citations

Oct 31, 2019·Molecular Biology and Evolution·Melissah RoweSteve Dorus
Nov 12, 2020·Reproduction in Domestic Animals = Zuchthygiene·Carolyn J P JonesSandra Wilsher

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