Leflunomide induces immunosuppression in collagen-induced arthritis rats by upregulating CD4+CD25+ regulatory T cells

Canadian Journal of Physiology and Pharmacology
Ting-Yu WangQian Zhou

Abstract

This study was to investigate the effect of leflunomide on the immunosuppressive CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) in collagen-induced arthritis (CIA) rats. CIA was induced by collagen type II in Wistar rats. Immunofluorescence flow cytometry and RT-PCR were used to determine the proportion of CD4+CD25+ Tregs and the expression of Foxp3 mRNA, respectively. Proliferation of T lymphocytes was assayed with MTT reagent, and the level of transforming growth factor beta1 (TGF-beta1) in the supernatant of concanavalin A (Con A)-induced T lymphocytes was determined by ELISA kit. Our investigations demonstrated that inhibition of arthritis by leflunomide was related to changes in CD4+CD25+ Tregs. In addition, A771726, which is the active metabolite of leflunomide, promoted the differentiation of spleen lymphocytes into CD4+CD25+ Tregs, increased antiinflammatory cytokine TGF-beta1 secretion, and adjusted the activity of Con A-induced lymphocytes in vitro.

References

Jan 1, 1988·Clinical Immunology and Immunopathology·N InamuraM Kohsaka
Nov 23, 2000·British Journal of Pharmacology·S CuzzocreaA P Caputi
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adam P KohmStephen D Miller
Jan 11, 2003·Science·Shohei HoriShimon Sakaguchi
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Apr 6, 2005·The Journal of Experimental Medicine·Julien C MarieAlexander Y Rudensky
May 5, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Clare Baecher-AllanDavid A Hafler
Jun 30, 2005·Arthritis and Rheumatism·Mary E MorganRené E M Toes
Jul 1, 2005·Arthritis Research & Therapy·Michel P M VierboomBert 't Hart
Mar 16, 2006·Blood·Xavier ValenciaPeter E Lipsky
Jan 4, 2007·The Journal of Experimental Medicine·Suchita NadkarniMichael R Ehrenstein
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Song Guo ZhengDavid A Horwitz
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucía Gil-GuerreroJuan José Lasarte
Dec 4, 2008·The American Journal of Chinese Medicine·Ting-Yu WangJi-Hui Tang

❮ Previous
Next ❯

Citations

Nov 8, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Di JinYong Zhao
Jul 28, 2013·Current Rheumatology Reports·Faye A H CoolesAmy E Anderson
Nov 2, 2013·Journal of Medicinal Food·Orie YoshinariManashi Bagchi
Jan 16, 2016·Immunopharmacology and Immunotoxicology·Samra Sardar, Åsa Andersson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.