Left-handed DNA: from synthetic polymers to chromosomes

Journal of Biomolecular Structure & Dynamics
T M JovinF Eckstein

Abstract

The interconversions between right-handed (R) and left-handed (L) helical conformations of DNA have been assessed by spectroscopic, electrophoretic, immunochemical, and enzymatic techniques. We have screened salt and solvent conditions which facilitate these transitions, as well as certain chemical modifications of the bases and backbone of defined synthetic polynucleotides. These include major and minor groove substituents as well as phosphorothioate analogues of selected phosphodiester bonds. We have established: R-L transitions in poly[d(G-C)] with iodo, bromo, methyl, and aza substitutions at the C5 position of cytosine, or phosphorothioate modification of the dGpC linkage. R-L transitions in the [d(A-C).d(G-T)]n sequence family using polymers modified as in the case of poly[d(G-C)]. The isomerizations are highly salt and temperature dependent. a possible L form of poly[d(A-T)] substituted with 2-amino adenine. the immunogenicities of constitutive and facultative Z-DNAs. the recognition specificities of different anti-Z-DNA IgGs for the spectrum of available polynucleotide probes. Some IgGs are sequence-specific. stabilization by IgG of otherwise transient left-handed conformations. anti-Z-DNA IgG binding to acid-fixed poly...Continue Reading

References

Jan 1, 1978·Annual Review of Biophysics and Bioengineering·W R Bauer
May 1, 1979·Virology·D A Zarling, I Keshet
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D J PatelF M Pohl
Nov 1, 1979·Journal of Inorganic Biochemistry·D J SzaldaS J Lippard
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·F M PohlJ J Holbrook
Jun 1, 1972·The Journal of Cell Biology·D E Olins, A L Olins
Jan 1, 1972·Results and Problems in Cell Differentiation·M Lezzi, M Robert
Jan 12, 1973·Nature·E M BradburyH W Rattle
Dec 1, 1967·Die Naturwissenschaften·F M Pohl
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·A RichF Azorin
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·T O'ConnorR D Wells
Sep 14, 1981·FEBS Letters·B Malfoy, M Leng
Jan 1, 1982·CRC Critical Reviews in Biochemistry·R L Adams, R H Burdon
Dec 15, 1982·Journal of Molecular Biology·S M Taylor, P A Jones
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·E M LaferB D Stollar
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·M LengE Sage
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·M L PardueA Rich
Jun 1, 1983·Biopolymers·J Widom, R L Baldwin
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R M SantellaA Rich
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Behe, G Felsenfeld
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A NordheimA Rich
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C K SingletonR D Wells
Mar 3, 1983·Nature·R M WalmsleyT D Petes
Jun 9, 1983·Nature·G MorgeneggC C Kuenzle
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·T M JovinM Robert-Nicoud
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J C WangK Becherer
Mar 1, 1984·Journal of Biomolecular Structure & Dynamics·D A ZarlingT M Jovin
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·F D MillerJ B Rattner
Apr 21, 1983·Nature·D A Agard, J W Sedat
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J Arndt-JovinT M Jovin
Mar 11, 1983·Nucleic Acids Research·S ArnottR L Ratliff
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A Nordheim, A Rich
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M TomaszB D Stollar
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·H Castleman, B F Erlanger
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·V Sasisekharan
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·F D MillerJ H van de Sande

❮ Previous
Next ❯

Citations

Jul 1, 1991·Journal of Inorganic Biochemistry·D W Gruenwedel, M K Cruikshank
Jun 23, 2001·Biochimica Et Biophysica Acta·I KejnovskáM Vorlícková
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D M Soumpasis
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A ZarlingA H Zarling
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F LancillottiC Alonso
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M P KladdeJ Gorski
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Egli, R V Gessner
Mar 1, 1984·Journal of Biomolecular Structure & Dynamics·D A ZarlingT M Jovin
Aug 1, 1992·Journal of Biomolecular Structure & Dynamics·P MojzesP Miskovsky
Dec 1, 1994·Journal of Biomolecular Structure & Dynamics·T BechertD J Arndt-Jovin
May 1, 1985·The Journal of Cell Biology·F LancillottiB D Stollar
Aug 1, 1990·The Journal of Cell Biology·M O Soyer-GobillardE Delain
Jun 25, 1985·Nucleic Acids Research·J A Taboury, E Taillandier
Jan 10, 1986·Nucleic Acids Research·H MarcaudC Reiss
Jun 25, 1986·Nucleic Acids Research·S Devarajan, R H Shafer
Aug 26, 1986·Nucleic Acids Research·T J Thomas, R P Messner
Feb 25, 1987·Nucleic Acids Research·A NordheimA Rich
Sep 12, 1988·Nucleic Acids Research·M CollJ Igolen
Oct 25, 1990·Nucleic Acids Research·A T Ansevin, A H Wang
Oct 6, 1999·Genome Research·L N BullN B Freimer
Jan 1, 1989·International Reviews of Immunology·B D Stollar
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Dec 15, 1986·European Journal of Biochemistry·M DelepierreB P Roques
Oct 1, 1984·Bioscience Reports·F D MillerJ H van de Sande
Jan 1, 1985·Biochemistry·F D MillerJ H van de Sande

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.