PMID: 6410390Jul 1, 1983Paper

Left-handed Z-DNA in bands of acid-fixed polytene chromosomes

Proceedings of the National Academy of Sciences of the United States of America
D J Arndt-JovinT M Jovin

Abstract

Antibodies to DNA in the left-handed (Z) conformation bind to acid-fixed polytene chromosomes of both Chironomus thummi and Drosophila melanogaster, as shown by direct and indirect immunofluorescence. Comparison of the phase-contrast, immunofluorescence, and DNA staining patterns shows a predominant localization of the antibody to the regions of high contrast and DNA density, the bands. The immunofluorescence is completely abolished by competition with polynucleotides in the Z conformation but not by those in the B form. DNase but not RNase treatment eliminates the antibody staining. Actinomycin D inhibits binding, whereas mithramycin has no effect. The highly reproducible immunofluorescence patterns obtained with the anti-Z-DNA antibodies demonstrate variations in fluorescence intensity between particular bands, which can be quantitated by laser scanning and photon counting techniques. The telomeric regions and DNA strands associated with end-to-end chromosome linkage and ectopic pairing are exceptionally bright. At saturation, average values of 1 IgG molecule per 3,000 base pairs and 1 per 15,000 base pairs are found in the intensely and weakly staining regions, respectively. An alternative statement is that the left-handed Z...Continue Reading

Citations

Jan 1, 1986·CRC Critical Reviews in Biochemistry·B D Stollar
Aug 5, 1984·Nature·C LagravèreJ Laval
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matteo de RosaMaria Armenia Carrondo
Jan 1, 1989·International Reviews of Immunology·B D Stollar
Jul 3, 2003·Nature Reviews. Genetics·Alexander Rich, Shuguang Zhang
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·T M JovinF Eckstein
Mar 1, 1984·Journal of Biomolecular Structure & Dynamics·D A ZarlingT M Jovin
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·G Yagil
Nov 1, 1987·Quarterly Reviews of Biophysics·Z Kam
Aug 1, 1984·Journal of Cellular Physiology·U NymanN R Ringertz
Jun 1, 1990·Molecular and Cellular Biology·J C Ruiz, G M Wahl
May 11, 1993·Biochemistry·D J Arndt-JovinT M Jovin
Jun 1, 1992·Differentiation; Research in Biological Diversity·G M JagielloM B Ducayen-Knowles
Nov 15, 1985·Science·J L Marx

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.