Lefty-1 alleviates TGF-β1-induced fibroblast-myofibroblast transdifferentiation in NRK-49F cells

Drug Design, Development and Therapy
Lijun ZhangPin Wu

Abstract

Fibroblast activation and proliferation are important for fibroblast-myofibroblast transdifferentiation, a crucial process in the pathological changes that define renal interstitial fibrosis. The left-right determination factor (Lefty) is an important cytokine of the transforming growth factor (TGF)-β family, with two variants, Lefty-1 and Lefty-2, in mice. Lefty has diverse functions, such as the regulation of embryonic development, the inhibition of TGF-β1 signaling, and the suppression of tumor activity. However, whether Lefty-1 influences fibroblast activation and proliferation, and consequently prevents fibroblast-myofibroblast transdifferentiation, remains unclear. This study aimed to investigate whether Lefty-1 can attenuate TGF-β1-induced fibroblast-myofibroblast transdifferentiation in normal rat kidney interstitial fibroblast cells (NRK-49F), as well as the mechanisms underlying any effects. Results showed that the typical fibroblast cell morphology of NRK-49F cells was altered after TGF-β1 treatment and that Lefty-1 significantly prevented this change in a dose-dependent manner. Further analyses demonstrated decreased proliferating cell nuclear antigen, cyclin D1, collagen I(A1), alpha-smooth muscle actin, and fibron...Continue Reading

Citations

Dec 30, 2017·International Journal of Molecular Medicine·Lijun ZhangJie Zhang
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mi-Gyeong GwonKwan-Kyu Park
Jan 8, 2021·Journal of Cardiovascular Translational Research·Chang-Yi LiSong-Nan Li
Aug 27, 2021·Cellular Signalling·Alexis Paulina Jiménez-UribeJosé Pedraza-Chaverri
Jan 22, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yang YiYingdan Zhao

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Methods Mentioned

BETA
antisense oligonucleotides
electrophoresis
PCR

Software Mentioned

MODFIT
SPSS

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