Leghemoglobin-derived radicals. Evidence for multiple protein-derived radicals and the initiation of peribacteroid membrane damage.

The Journal of Biological Chemistry
S MoreauA Puppo

Abstract

Reaction of H2O2 with ferric leghemoglobin (metLb, the monomeric, oxygen-carrying, heme protein from root nodules of nitrogen-fixing plants) has been previously shown to generate an iron(IV)-oxo (ferryl) species and at least one protein radical. The latter has been suggested to be a tyrosine-derived phenoxyl radical present at Tyr-133 in the soybean protein and Tyr-138 in the lupin protein. To obtain further information on these protein radicals and their potential interaction with the physiologically important peribacteroid membrane (which surrounds the microsymbiont in vivo), EPR spin trapping studies have been carried out with soybean metLb. Evidence has been obtained for at least two additional protein-derived radicals in addition to the phenoxyl radical; these radicals are transient and reactive in nature. These species are carbon-centered, and at least one is a tertiary species (.CR1R2R3); these radicals may be side chain- or alpha-carbon-derived, their exact sites have not been determined. Some of these radicals are on the protein surface and may be key intermediates in the formation of protein dimers. These radicals have been shown to be capable of reacting with peribacteroid membrane fractions, with the consequent gene...Continue Reading

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Citations

Apr 15, 2004·Free Radical Biology & Medicine·Michael J Davies, Clare L Hawkins
Aug 8, 2001·European Journal of Biochemistry·R E DiederixG W Canters
Jan 14, 2010·Prikladnaia biokhimiia i mikrobiologiia·O V Kosmachevskaia, A F Topunov
Nov 4, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Susanna Herold, Alain Puppo
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Sep 23, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Seok-Jong SuhCheorl-Ho Kim
Apr 22, 2011·Meat Science·Renata S FilguerasVéronique Santé-Lhoutellier
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Feb 23, 2010·Antioxidants & Redox Signaling·Brandon J Reeder
Jul 27, 1999·Journal of Bacteriology·R SantosD Touati

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