Legionella pneumophila carrying the virulence-associated lipopolysaccharide epitope possesses two functionally different LPS components

Microbiology
Katja ReichardtJ H Helbig

Abstract

Phase-variable expression of Legionella pneumophila lipopolysaccharide (LPS) has not been described in detail for strains possessing the virulence-associated epitope recognized by the monoclonal antibody (mAb) 3/1 of the Dresden Panel. About 75 % of cases of community-acquired legionellosis are caused by mAb 3/1-positive strains. In this study, the LPS architecture of the mAb 3/1-positive Corby strain was investigated during its life cycle in broth culture and inside monocytic host cells. During the exponential growth phase in broth, the highly acetylated and therefore strongly hydrophobic mAb 3/1 epitope is expressed continuously, but only 3 % of the bacteria can be detected using mAb 59/1, which recognizes a short-chain variant of the Legionella LPS that is less hydrophobic due to missing acetylations of the O-chain. The percentage of mAb 59/1-positive legionellae increases up to 34 % in the post-exponential growth phase. LPS shed in broth during the exponential phase is mAb 59/1-negative, and mAb 3/1-positive components do not possess short-chain molecules. The LPS pattern expressed and shed inside U937 cells and A/J mouse macrophages points to the same regulatory mechanisms. During the so-called 'pregnant pause', the period...Continue Reading

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Citations

Oct 29, 2010·Journal of Clinical Microbiology·Mike VergnesDominique Schneider
Nov 1, 2013·Chemical & Pharmaceutical Bulletin·Yuko KawasakiYutaka Hirakura
Jan 11, 2020·Frontiers in Microbiology·Marta Palusinska-SzyszBozena Kowalczyk

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