PMID: 9420857May 1, 1995Paper

Leiomyoma primary cultures have elevated transcriptional response to estrogen compared with autologous myometrial cultures

Journal of the Society for Gynecologic Investigation
J AndersenR J Miksicek

Abstract

We tested the hypothesis that uterine leiomyomas are hypersensitive to estrogen as compared with autologous human myometrium. The estrogen-induced transcriptional responses of uterine leiomyoma and myometrial primary cultures were determined by transient expression assays. The relative levels of estrogen receptor (ER) in myometrial and leiomyoma tissues were determined by immunoblot. Myometrial and leiomyoma primary cultures were transcriptionally responsive to the estrogen ethinyl estradiol (eE2). The partial agonist tamoxifen did not elicit a positive transcriptional response and antagonized estrogen-induced transcription in the cultured cells. The responses of hormone-treated leiomyoma cells averaged 4.5-fold higher than those in controls with no hormone (P = .0001). The myometrial cells from women in the follicular phase exhibited little if any transcriptional response to eE2, whereas myometrial cells from women in the luteal phase had a transcriptional response to eE2 averaging threefold higher than that in no-hormone controls (P = .0083). Differences in response between autologous myometrial and leiomyoma cultures were statistically significant by the two-tailed Wilcoxon paired nonparametric signed-rank test (n = 11; P = ...Continue Reading

Citations

Oct 19, 2002·Molecular and Cellular Endocrinology·Leslie C HodgesCheryl L Walker
Apr 9, 2010·Endocrinology·Hiroshi IshikawaTakeshi Kurita
Jun 27, 2003·Environmental Health Perspectives·Gordon P FlakeDarlene Dixon
Jun 7, 2008·Best Practice & Research. Clinical Obstetrics & Gynaecology·Stanley Okolo
Apr 1, 2008·Best Practice & Research. Clinical Obstetrics & Gynaecology·Essam-Eldin R Othman, Ayman Al-Hendy
Mar 1, 2006·Obstetrics and Gynecology Clinics of North America·Pavna K BrahmaGregory M Christman
Mar 18, 2005·Cancer Genetics and Cytogenetics·Avery A Sandberg
Oct 21, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Virgil S Bideau, Angela T Alleyne
Feb 6, 2008·Fertility and Sterility·Sandro G HilárioEdmund C Baracat
Jun 12, 2009·Reproductive Sciences·Guangli SuoJean Y J Wang
Mar 20, 1999·Biology of Reproduction·R C BurghardtJ Andersen
Jun 25, 2015·Current Opinion in Obstetrics & Gynecology·Molly B Moravek, Serdar E Bulun
Jun 17, 1999·Clinical Obstetrics and Gynecology·R L Barbieri
May 20, 2020·Journal of Clinical Medicine·Michał CiebieraAyman Al-Hendy
Sep 22, 2018·The Journal of Clinical Endocrinology and Metabolism·Sinnie Sin Man NgJames H Segars
May 14, 2010·Systems Biology in Reproductive Medicine·Yong JiangJean Y J Wang
Apr 2, 2019·Fertility and Sterility·Malcolm G Munro

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