Leishmania aethiopica: strain identification and characterization of superoxide dismutase-B genes

Experimental Parasitology
Abebe GenetuL Gedamu

Abstract

This study was performed to characterize the genes that code for superoxide dismutase (SOD) in Leishmania aethiopica. It involved three main steps: specimen collection and parasite isolation, species identification, and molecular characterization of the SOD genes. Out of 20 skin slit specimens cultured and processed from suspected cutaneous leishmaniasis patients enrolled in the study, five (25%) were found to be positive for motile promastigotes. Isoenzyme electrophoresis and PCR-RFLP results confirmed that the isolates were L. aethiopica. Superoxide dismutase-B (SODB) genes were identified from L. aethiopica for the first time. Iron superoxide dismutase-B genes amplified from promastigotes of L. aethiopica (LaeFeSODB) were similar in size to the SODB genes of other Leishmania species. Nucleotide sequences of LaeFeSODB1 showed 95.4, 93.5, and 97.3% identity with L. donovani SODB1 (LdFeSODB1) L. major SODB1 (LmFeSODB1) and L. tropica SODB1 (LtrFeSODB1), respectively. Similarly, LaeFeSODB2 showed 95.9 and 94.1 and 97.6% identity with LdFeSODB2 and LmFeSODB2 and LtrFeSODB2, respectively. On the other hand, predicted amino acid sequence comparison indicated that LaeFeSODB1 had 91.3, 89.8, and 93.9% identity with LdFeSODB1, LmFeSOD...Continue Reading

References

Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·S M Le BlancqW Peters
Aug 1, 1996·Current Opinion in Immunology·C BogdanM Röllinghoff
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Sep 23, 2003·Infection and Immunity·Katherine A PlewesLashitew Gedamu
Jun 1, 1990·Parasitology Today·C BogdanW Solbach

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Citations

Dec 12, 2007·Cellular Microbiology·Chau Huynh, Norma W Andrews
May 28, 2011·Experimental Parasitology·Teklu KuruAbraham Aseffa
May 31, 2011·Free Radical Biology & Medicine·Tim Van AsschePaul Cos
Jul 2, 2008·Memórias do Instituto Oswaldo Cruz·Héctor MateoManuel Sánchez-Moreno

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