Abstract
CBA/J mice are resistant to Leishmania major and susceptible to Leishmania amazonensis. Early events determine infection outcome. Until now, PIV (in vitro priming) immune response to L. amazonensis has not been assessed. Herein, we have shown that compared to L. major, L. amazonensis induced higher parasite burden associated to similar IL-4, IFN-gamma, and TNF-alpha mRNA expressions and IFN-gamma and IL-10 levels. Although similar amounts of IL-10 were detected, the frequency of intracellular IL-10 positive B cells was enhanced in spleen cells stimulated with anti-CD3/anti-CD28, or anti-CD3/anti-CD28 and L. amazonensis, compared to L. major-stimulation. Interestingly, IL-10- producing B cells were reduced in response to anti-CD3/anti-CD28 stimulation combined with L. major compared to the other groups. L. amazonensis may favor T regulatory cell development, since 40% of all the CD4+CD25+ were CD25(high) cells. These data suggest that in PIV, susceptibility to L. amazonensis is not related to Th cell polarization, but to the presence and activity of regulatory T and B cells.
References
Jan 1, 1990·The Journal of Experimental Medicine·M D SadickR M Locksley
Aug 1, 1995·European Journal of Immunology·T LaskayW Solbach
Jul 1, 1993·The Journal of Experimental Medicine·A H Shankar, R G Titus
Nov 1, 1996·Experimental Parasitology·V PalanivelD A Harn
Feb 14, 1998·Immunity·A DiefenbachC Bogdan
Nov 11, 1999·Journal of Immunological Methods·E O'ConnorJ D Davies
Feb 13, 2001·Microbes and Infection·V Lemos de SouzaL A Rodrigues de-Freitas
Jul 12, 2001·Infection and Immunity·R G TitusM B Soares
Nov 5, 2002·Current Opinion in Immunology·Maria A Curotto de Lafaille, Juan J Lafaille
Jan 30, 2003·Journal of Leukocyte Biology·David M Mosser
Apr 23, 2003·Microbes and Infection·Ivana Nunes GomesPatrícia Sampaio Tavares Veras
Jul 23, 2003·Infection and Immunity·Jiaxiang JiLynn Soong
Jun 24, 2004·The Journal of Experimental Medicine·Petr BocekWilliam E Paul
Jun 29, 2004·Journal of Medical Entomology·E Nieves, P F Pimenta
Mar 9, 2005·The Journal of Experimental Medicine·Ruka SetoguchiShimon Sakaguchi
Oct 15, 2010·Memórias do Instituto Oswaldo Cruz·Rafaella F Q GrenfellSimone A Rezende
Citations
Nov 16, 2012·PloS One·Antonio Luis de Oliveira Almeida PetersenPatrícia Sampaio Tavares Veras
Jul 5, 2013·PLoS Pathogens·Tobias SchwarzHeidrun Moll
Sep 6, 2014·PloS One·Katherine N Gibson-CorleyDouglas E Jones
Dec 5, 2008·Veterinary Immunology and Immunopathology·Cleusa Alves Theodore RodriguesPatrícia Sampaio Tavares Veras
Feb 1, 2012·Clinical and Experimental Immunology·A B B MacedoP M De Luca
May 6, 2016·Frontiers in Immunology·Paula Mello De Luca, Amanda Beatriz Barreto Macedo
Nov 20, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oleg E AkilovMei X Wu