Leishmania amazonensis: participation of regulatory T and B cells in the in vitro priming (PIV) of CBA/J spleen cells susceptible response

Experimental Parasitology
P S T VerasLuiz Antônio Rodrigues de Freitas

Abstract

CBA/J mice are resistant to Leishmania major and susceptible to Leishmania amazonensis. Early events determine infection outcome. Until now, PIV (in vitro priming) immune response to L. amazonensis has not been assessed. Herein, we have shown that compared to L. major, L. amazonensis induced higher parasite burden associated to similar IL-4, IFN-gamma, and TNF-alpha mRNA expressions and IFN-gamma and IL-10 levels. Although similar amounts of IL-10 were detected, the frequency of intracellular IL-10 positive B cells was enhanced in spleen cells stimulated with anti-CD3/anti-CD28, or anti-CD3/anti-CD28 and L. amazonensis, compared to L. major-stimulation. Interestingly, IL-10- producing B cells were reduced in response to anti-CD3/anti-CD28 stimulation combined with L. major compared to the other groups. L. amazonensis may favor T regulatory cell development, since 40% of all the CD4+CD25+ were CD25(high) cells. These data suggest that in PIV, susceptibility to L. amazonensis is not related to Th cell polarization, but to the presence and activity of regulatory T and B cells.

References

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Citations

Nov 16, 2012·PloS One·Antonio Luis de Oliveira Almeida PetersenPatrícia Sampaio Tavares Veras
Dec 5, 2008·Veterinary Immunology and Immunopathology·Cleusa Alves Theodore RodriguesPatrícia Sampaio Tavares Veras
Feb 1, 2012·Clinical and Experimental Immunology·A B B MacedoP M De Luca
May 6, 2016·Frontiers in Immunology·Paula Mello De Luca, Amanda Beatriz Barreto Macedo
Nov 20, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oleg E AkilovMei X Wu

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