Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function

Parasite Immunology
Thiago M CardosoEdgar M de Carvalho

Abstract

The polymorphism observed in Leishmania braziliensis is associated with different clinical forms of leishmaniasis. Neutrophils (PMNs) participate in the pathogenesis of leishmania infection, and here, we evaluate neutrophil function after infection with isolates of L. braziliensis from cutaneous leishmaniasis (CL) or disseminated leishmaniasis (DL) patients. Neutrophils from 30 healthy subjects (HS) were infected with isolates of L. (V.) braziliensis obtained from three CL and three DL patients. They were infected at the ratio of 3:1 parasites per neutrophil, and leishmania uptake was evaluated by microscopy. The neutrophil activation markers and oxidative burst by expression of dihidrorhodamine (DHR) were evaluated by flow cytometry and cytokine production by ELISA. The frequency of infected cells and the number of amastigotes were higher in neutrophils infected with CL isolates compared to DL isolates (P < 0.05). The DHR and CD66b expression after infection with DL isolate was lower than with CL isolates. There was no difference regarding chemokine production. The L. (V.) braziliensis isolates of DL induced lower respiratory burst and neutrophils activation markers compared with CL isolates which may contribute to parasite su...Continue Reading

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Citations

Apr 16, 2020·Parasite Immunology·Gabriela Pessenda, João Santana da Silva
Aug 25, 2020·Journal of Tropical Pediatrics·Kovy Arteaga-LiviasAlfonso J Rodriguez-Morales
Dec 25, 2019·Journal of Immunology Research·Áurea GabrielGabriela Santos-Gomes
Mar 19, 2021·Frontiers in Immunology·Katiuska PasselliFabienne Tacchini-Cottier
Jun 26, 2021·Frontiers in Cellular and Infection Microbiology·Greta VolpedoAbhay R Satoskar
Aug 24, 2021·Emerging Infectious Diseases·Anastácio Q SousaMargarida M L Pompeu

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Methods Mentioned

BETA
biopsy
flow cytometry
PMA
FACS
ELISA

Software Mentioned

Flow Jo

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