Leishmania braziliensis: localization of glycoproteins in promastigotes

Experimental Parasitology
K NagakuraT Nakae

Abstract

Two species of glycoproteins from Leishmania braziliensis promastigotes of apparent molecular weights of 53,000 (glycoprotein 53) and 47,000 (glycoprotein 47) were localized. Four lectins with different sugar specificities bound to the blotting sheet to which the electrophoretically separated materials were transferred. Concanavalin A and Ricinus communis agglutinin bound to the band of glycoprotein 53 and the lectin from Dolichos biflorus bound to the band of glycoprotein 47. Wheat germ agglutinin bound to the bands of both glycoproteins. Histochemical examinations using fluorescence labeled lectins demonstrated that the glycoproteins 53 and 47 were located on the cell surface and in the cytoplasm of promastigotes, respectively. The results are consistent with the result of agglutination test.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1977·Experimental Parasitology·D M Dwyer
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Sep 15, 1972·Science·N Sharon, H Lis
Aug 1, 1983·Molecular and Biochemical Parasitology·P R Gardiner, D M Dwyer
Jan 13, 1984·Biochemical and Biophysical Research Communications·A J ParodiJ C Engel
Aug 1, 1981·The Journal of protozoology·T I Doran, R Herman

❮ Previous
Next ❯

Citations

Apr 1, 1987·Experimental Parasitology·M CuperlovićL Hajduković
Jul 2, 2009·Parasitology International·Patrícia K S RamosWanderley de Souza
Jun 12, 2008·Revista Da Sociedade Brasileira De Medicina Tropical·Adriano Gomes-SilvaMarcelo Simão Ferreira
Jul 1, 1990·Clinical Microbiology Reviews·M Slifkin, R J Doyle

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.