Leishmania donovani: effect of verapamil on in vitro susceptibility of promastigote and amastigote stages of Indian clinical isolates to sodium stibogluconate

Experimental Parasitology
Ranjini ValiathanNancy Malla

Abstract

Although pentavalent antimonials are the first-line drug for treatment of visceral leishmaniasis all over the world, yet, in India, increasing number of patients are being reported to be unresponsive to sodium stibogluconate. Verapamil, a calcium channel blocker, affects drug uptake by preventing its efflux and thereby accumulation within the cell. In the present study, effect of verapamil on in vitro susceptibility of both promastigote and amastigote stages of 15 clinical isolates and standard strain of Leishmania donovani to sodium stibogluconate was evaluated by detection of acid phosphatase. Amastigotes were found more susceptible to sodium stibogluconate than the promastigotes (p<0.05) and in the presence of verapamil, IC(50) value of sodium stibogluconate was reduced only for those isolates, which had a higher IC(50). Verapamil alone did not have any effect on the parasites. The results indicate that amastigotes are more susceptible to sodium stibogluconate than promastigotes and verapamil can reverse the in vitro drug resistance of L. donovani clinical isolates to sodium stibogluconate.

References

Nov 1, 1992·Transactions of the Royal Society of Tropical Medicine and Hygiene·C P ThakurB B Verma
Mar 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·R A NealM Iwobi
Oct 1, 1988·Experimental Parasitology·J D Berman, M Grogl
Mar 1, 1985·Somatic Cell and Molecular Genetics·S AkiyamaM M Gottesman
Oct 8, 1999·Lancet·B L Herwaldt
Oct 26, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S SundarH W Murray
Oct 26, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S SundarH W Murray
Nov 13, 2001·Tropical Medicine & International Health : TM & IH·S Sundar
Oct 17, 2002·Journal of Biosciences·Anindita BhattacharyyaSwadesh Duttagupta
Feb 8, 2013·The American Journal of Tropical Medicine and Hygiene·Max GroglRichard J Sciotti

❮ Previous
Next ❯

Citations

Oct 16, 2013·Antimicrobial Agents and Chemotherapy·Alex G PenicheBruno L Travi
Nov 6, 2009·Journal of Biomedicine & Biotechnology·Helena C Maltezou
Oct 14, 2016·Frontiers in Pharmacology·Jose A Garcia-SalcedoMiguel Soriano
Jan 14, 2012·Memórias do Instituto Oswaldo Cruz·Juliana Quero Reimão, André Gustavo Tempone
Jul 1, 2020·Antimicrobial Agents and Chemotherapy·M Van den KerkhofG Caljon
Apr 11, 2018·Frontiers in Chemistry·Anna MeierEric Beitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.