Leishmania donovani requires functional Cdc42 and Rac1 to prevent phagosomal maturation.

Infection and Immunity
Maria LermBirgitta Rasmusson

Abstract

Leishmania donovani promastigotes survive inside macrophage phagosomes by inhibiting phagosomal maturation. The main surface glycoconjugate on promastigotes, lipophosphoglycan (LPG), is crucial for survival and mediates the formation of a protective shell of F-actin around the phagosome. Previous studies have demonstrated that this effect involves inhibition of protein kinase C alpha. The present study shows that functional Cdc42 and Rac1 are required for the formation of F-actin around L. donovani phagosomes. Moreover, we present data showing that phagosomes containing LPG-defective L. donovani, which is unable to induce F-actin accumulation, display both elevated levels of periphagosomal F-actin and impaired phagosomal maturation in macrophages with permanently active forms of Cdc42 and Rac1. We conclude that L. donovani engages Cdc42 and Rac1 to build up a protective coat of F-actin around its phagosome to prevent phagosomal maturation.

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Citations

Feb 20, 2008·Genome Biology·J Maxwell SilvermanNeil E Reiner
Jul 16, 2011·International Journal of Cell Biology·Luisa Jordao, Otilia V Vieira
Feb 17, 2007·Molecular and Biochemical Parasitology·Claire J EscaronEmmanuelle Caron
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Apr 2, 2013·Journal of Leukocyte Biology·Rachel PolandoMary Ann McDowell
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria LermEva Särndahl
Sep 27, 2019·Frontiers in Cellular and Infection Microbiology·Tamara da Silva VieiraAlbert Descoteaux
Jun 6, 2020·Journal of Cell Science·Mathieu B PoirierRene E Harrison
Mar 24, 2021·Parasite Immunology·Supratim PradhanBudhaditya Mukherjee

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