Abstract
Establishment of infection by Leishmania depends on the transformation of the invading metacyclic promastigotes into the obligatory intracellular amastigotes, and their subsequent survival in the macrophage phagolysosome, which is low in magnesium. We show that two Leishmania major proteins designated MGT1 and MGT2, which play a critical role in these processes, belong to the two-transmembrane domain (2-TM-GxN) cation transporter family and share homology with the major bacterial magnesium transporter CorA. Although both are present in the endoplasmic reticulum throughout the life cycle of the parasite, MGT1 is more highly expressed in the infectious metacyclic parasites, while MGT2 is enriched in the immature procyclic stages. The two proteins, although predicted to be structurally similar, have features that suggest different regulatory or gating mechanisms. The two proteins may also be functionally distinct, since only MGT1 complements an Escherichia coli DeltaCorA mutant. In addition, deletion of one mgt1 allele from L. major led to increased virulence, while deletion of one allele of mgt2 resulted in slower growth and total loss of virulence in vitro and in vivo. This loss of virulence may be due to an impaired transformat...Continue Reading
References
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A CruzS M Beverley
Apr 1, 1996·Bioscience Reports·D L GillA Alfonso
Apr 16, 1998·Microbiology·Tao TaoMichael E Maguire
Jun 11, 1998·Molecular Microbiology·R L Smith, M E Maguire
Oct 17, 1998·Microbial & Comparative Genomics·D G KehresM E Maguire
Jul 10, 1999·The Journal of Biological Chemistry·D M BuiR J Schweyen
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Mar 30, 2001·The Journal of Biological Chemistry·A GraschopfR J Schweyen
Jun 13, 2001·Genomics·G ZsurkaR J Schweyen
Jun 23, 2001·Journal of Molecular Biology·J ShiK Mizuguchi
Dec 21, 2002·Molecular Microbiology·Sofia ErikssonJay C D Hinton
May 20, 2003·Current Opinion in Plant Biology·Richard C Gardner
Dec 12, 2003·Bioinformatics·András Fiser, Andrej Sali
Dec 12, 2003·Genes and Immunity·C M ElsoE Handman
Nov 2, 2004·Biological Research·Henrique LanzaMaria A Souza
Jun 7, 2005·International Journal for Parasitology·James StewartEmanuela Handman
Sep 24, 2005·Molecular Genetics and Genomics : MGG·Volker KnoopKatrin Weyand
Apr 7, 2006·Nature·Vladimir V LuninChristopher M Koth
Jul 11, 2006·Current Opinion in Structural Biology·Michael E Maguire
Jul 22, 2006·Science·Said EshaghiPär Nordlund
Aug 12, 2006·The EMBO Journal·Jian Payandeh, Emil F Pai
Sep 22, 2006·Infection and Immunity·Kendra E Maloney, Miguel A Valvano
Oct 3, 2006·International Journal for Parasitology·Alessandro D UboldiEmanuela Handman
Feb 6, 2007·Parasitology·L KedzierskiE Handman
Apr 10, 2007·Trends in Microbiology·Eric Alix, Anne-Béatrice Blanc-Potard
Jul 11, 2007·Cellular and Molecular Life Sciences : CMLS·D Niegowski, S Eshaghi
Aug 22, 2007·Molecular Membrane Biology·Krisztina M Papp-Wallace, Michael E Maguire
Citations
Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Albert GuskovSaid Eshaghi
May 31, 2012·Genome Research·Michael E ZwickTimothy D Read
Jun 15, 2011·Infection and Immunity·Brian C RussoGerard J Nau
Jul 11, 2013·PloS One·Paul CapewellKeith R Matthews
May 15, 2013·Journal of Proteomics·Raja Sekhar NirujogiAkhilesh Pandey
Jul 6, 2011·Molecular Plant Pathology·Caleb M KerseyC Korsi Dumenyo
Jun 9, 2015·Biochimica Et Biophysica Acta·Robert M CleverleyStephen M Prince
Mar 17, 2015·Biochimica Et Biophysica Acta·Sumio IshijimaIkuko Sagami
Feb 22, 2013·The Biochemical Journal·Nurhuda NordinSaid Eshaghi
Aug 21, 2013·Biochimica Et Biophysica Acta·Jian PayandehEmil F Pai
Sep 13, 2012·PloS One·Jaya SahniAndrew M Scharenberg
May 17, 2017·Infection and Immunity·Sabine LeithererDidier Soulat
Dec 22, 2017·Emerging Topics in Life Sciences·Snezhana Akpunarlieva, Richard Burchmore
Jan 16, 2018·Journal of Proteome Research·Armando JardimChristoph H Borchers