Leishmania major: genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer

Acta Tropica
Mahnaz TashakoriAlimohammadian Mohammad Hossein

Abstract

Protozoan parasites of Leishmania major are the causative agents of cutaneous leishmaniasis in different parts of Iran. We applied PCR-based methods to analyze L. major parasites isolated from patients with active lesions from different geographic areas in Iran in order to understand DNA polymorphisms within L. major species. Twenty-four isolates were identified as L. major by RFLP analysis of the ribosomal internal transcribed spacer 1 (ITS1) amplicons. These isolates were further studied by single-strand conformation polymorphism (SSCP) analysis and sequencing of ITS1 and ITS2. Data obtained from SSCP analysis of the ITS1 and ITS2 loci revealed three and four different patterns among all studied samples, respectively. Sequencing of ITS1 and ITS2 confirmed the results of SSCP analysis and showed the potential of the PCR-SSCP method for assessing genetic heterogeneity within L. major. Different patterns in ITS1 were due to substitution of one nucleotide, whereas in ITS2 the changes were defined by variation in the number of repeats in two polymorphic microsatellites. In total five genotypic groups LmA, LmB, LmC, LmD and LmE were identified among L. major isolates. The most frequent genotype, LmA, was detected in isolates collec...Continue Reading

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Citations

Feb 21, 2007·Parasitology Research·Kishor PandeyHiroji Kanbara
Jul 8, 2011·The American Journal of Tropical Medicine and Hygiene·Pieter-Paul A M van ThielAldert Bart
Jul 6, 2012·The American Journal of Tropical Medicine and Hygiene·Mohammad H AlimohammadianFarrokh Modabber
Jul 2, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Asad MirzaeiGabriele Schönian
Jun 17, 2014·Experimental Parasitology·Mohammad Amin GhateeHossein Mirhendi
Jun 15, 2011·Microbes and Infection·Tashakori MahnazGabriele Schönian
Jul 6, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mohammad Hossein AlimohammadianEbrahim Torkabadi
Sep 6, 2012·Tropical Medicine & International Health : TM & IH·Hamid Mahmoudzadeh-NiknamMohammad H Alimohammadian
Mar 27, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yan-Zi WenDe-Hua Lai
Apr 12, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mahdi FakharMohammad Amin Ghatee
May 4, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Hanene AttiaDhafer Laouini
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Mar 21, 2017·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Farzaneh KavarizadehMehdi Zarean
Jun 9, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Raziyeh Zeinvand LorestaniShokrollah Salmanzadeh
Jun 15, 2020·Acta Tropica·Mouad Ait KbaichMeryem Lemrani
Sep 13, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mansoure HosseiniAli Khamesipour
Aug 21, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Mohammed Hassan FlaihKhwam Reissan Hussein
Jun 2, 2020·Infectious Disorders Drug Targets·Sajjad PanahiJahangir Abdi

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