LeMAN4 endo-beta-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase.

Planta
R SchröderRoss G Atkinson

Abstract

Mannan transglycosylases are cell wall enzymes able to transfer part of the mannan polysaccharide backbone to mannan-derived oligosaccharides (Schröder et al. in Planta 219:590-600, 2004). Mannan transglycosylase activity was purified to near homogeneity from ripe tomato fruit. N-terminal sequencing showed that the dominant band seen on SDS-PAGE was identical to LeMAN4a, a hydrolytic endo-beta-mannanase found in ripe tomato fruit (Bewley et al. in J Exp Bot 51:529-538, 2000). Recombinant LeMAN4a protein expressed in Escherichia coli exhibited both mannan hydrolase and mannan transglycosylase activity. Western analysis of ripe tomato fruit tissue using an antibody raised against tomato seed endo-beta-mannanase revealed four isoforms present after 2D-gel electrophoresis in the pH range 6-11. On separation by preparative liquid isoelectric focussing, these native isoforms exhibited different preferences for transglycosylation and hydrolysis. These results demonstrate that endo-beta-mannanase has two activities: it can either hydrolyse mannan polysaccharides, or in the presence of mannan-derived oligosaccharides, carry out a transglycosylation reaction. We therefore propose that endo-beta-mannanase should be renamed mannan transgly...Continue Reading

References

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Citations

Aug 10, 2006·Functional & Integrative Genomics·Joshua S YuanFeng Chen
May 4, 2012·Journal of Experimental Botany·María del Carmen Rodríguez-GacioAngel J Matilla
Sep 21, 2012·BMC Evolutionary Biology·Henrik AspeborgBernard Henrissat
Dec 22, 2012·BMC Plant Biology·João Paulo FabiJoão Roberto Oliveira do Nascimento
Aug 24, 2013·BMC Research Notes·Carolina Aguilera-GálvezRicardo Acuña-Zornosa
Nov 30, 2013·International Journal of Molecular Sciences·Duoduo WangXuewu Duan
Apr 23, 2014·Carbohydrate Polymers·Sayani RayMarc Lahaye
Jun 5, 2012·Journal of Plant Physiology·Roneel PrakashRoswitha Schröder
Jul 28, 2010·The Plant Journal : for Cell and Molecular Biology·Susan E MarcusJ Paul Knox
Feb 3, 2015·Journal of Integrative Plant Biology·Lenka Franková, Stephen C Fry
Jun 7, 2015·International Journal of Biological Macromolecules·Sun-Li ChongMaija Tenkanen
Aug 21, 2013·Journal of Experimental Botany·Lenka Franková, Stephen C Fry
Nov 22, 2017·BMC Plant Biology·Marta MarmiroliNelson Marmiroli
Apr 24, 2018·Applied Microbiology and Biotechnology·Johan MorrillHenrik Stålbrand
May 11, 2017·BMC Plant Biology·Roneel PrakashRoswitha Schröder
Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Barbora StratilováMaria Hrmova
Sep 22, 2021·The Plant Journal : for Cell and Molecular Biology·Lenka Franková, Stephen C Fry

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