Abstract
A zebrafish ortholog of human lengsin was identified by EST analysis of an adult lens cDNA library. During zebrafish development, lengsin transcription is first detected at 24 h post-fertilization (hpf). Immunolocalization, using polyclonal antiserum generated against a Lengsin bacterial fusion protein, detects lens-specific protein in whole-mount embryos at 30 hpf. Lengsin expression in zebrafish follows the temporal expression of the alphaA- alphaB1- and betaB1-crystallin proteins in the lens. At 72 hpf, Lengsin is localized to a subpopulation of differentiating secondary fiber cells, while no expression is detected in the lens epithelial cells or central lens fibers. In the adult lens, Lengsin is restricted to a narrow band of cortical fibers and co-localizes with actin at the lateral faces of these interdigitating cells. Stable transgenic lines, using a 3 kb lengsin genomic fragment to regulate EGFP expression, recapitulate the Lengsin temporal and spatial expression patterns. Lengsin function in zebrafish lens formation was examined by antisense morpholino-mediated translation and mRNA splice inhibition. At 72 hpf, the lengsin morphant lenses are reduced in size and exhibit separations within the cortex due to defects in s...Continue Reading
References
Feb 1, 1992·Seminars in Cell Biology·D A Goodenough
Aug 1, 1980·The Journal of Cell Biology·D A GoodenoughJ E Lyons
Dec 1, 1995·The Journal of Biological Chemistry·V TaylorU Suter
Jun 21, 1994·Biochemistry·L F MicheaN Lagos
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·J ReizerM H Saier
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jun 17, 1998·Experimental Eye Research·J F HessP G FitzGerald
Feb 5, 1999·Gene·K KawakamiA Shima
Jun 1, 1999·Visual Neuroscience·T S VihtelicD R Hyde
May 24, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Z LiS S Easter
Oct 4, 2000·Nature Genetics·A Nasevicius, S C Ekker
Jul 31, 2001·Genesis : the Journal of Genetics and Development·S C Ekker, J D Larson
Dec 19, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T S VihtelicD R Hyde
Mar 7, 2002·Experimental Eye Research·Steven Bassnett
Jun 1, 1964·Experimental Eye Research·R J KAISER, D M MAURICE
Oct 11, 2003·Experimental Eye Research·Angus C GreyPaul J Donaldson
Apr 15, 2004·Developmental Biology·Xiangyun WeiDavid R Hyde
Apr 5, 2005·Mechanisms of Development·Xiaohai ShiThomas S Vihtelic
Jun 25, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ryan ThummelAlan R Godwin
Jan 20, 2006·The FEBS Journal·Amber A SmithMason Posner
Sep 12, 2006·Mechanisms of Development·Xiaohai ShiThomas S Vihtelic
Oct 3, 2006·Biochemical and Biophysical Research Communications·Francesca GrassiSimone Ottonello
Dec 13, 2006·Structure·Keith WyattGraeme Wistow
May 1, 2007·Experimental Eye Research·Ralf DahmGijs F J M Vrensen
May 11, 2007·Experimental Cell Research·Ming-Der PerngRoy A Quinlan
Jun 15, 2007·Developmental Neurobiology·Sean C KassenDavid R Hyde
Jan 8, 2008·The Journal of Biological Chemistry·Keith WyattGraeme Wistow
Citations
Oct 26, 2012·Molecular Biology of the Cell·Julie M HayesJeffrey M Gross
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Jeremy D O'ConnellEdward M Marcotte
Jul 5, 2011·Experimental Eye Research·Taylor R MurphyDavid R Hyde
May 20, 2011·Developmental Neurobiology·Gaia GestriStephan C F Neuhauss
Dec 4, 2010·Developmental Biology·Rachel K TittleJeffrey M Gross
Nov 23, 2017·The Journal of Biological Chemistry·Sanjay MishraHassane S Mchaourab
Aug 19, 2021·IUBMB Life·Caiying LiuLixia Lu