Lengsin expression and function during zebrafish lens formation.

Experimental Eye Research
Rachel L HardingThomas S Vihtelic

Abstract

A zebrafish ortholog of human lengsin was identified by EST analysis of an adult lens cDNA library. During zebrafish development, lengsin transcription is first detected at 24 h post-fertilization (hpf). Immunolocalization, using polyclonal antiserum generated against a Lengsin bacterial fusion protein, detects lens-specific protein in whole-mount embryos at 30 hpf. Lengsin expression in zebrafish follows the temporal expression of the alphaA- alphaB1- and betaB1-crystallin proteins in the lens. At 72 hpf, Lengsin is localized to a subpopulation of differentiating secondary fiber cells, while no expression is detected in the lens epithelial cells or central lens fibers. In the adult lens, Lengsin is restricted to a narrow band of cortical fibers and co-localizes with actin at the lateral faces of these interdigitating cells. Stable transgenic lines, using a 3 kb lengsin genomic fragment to regulate EGFP expression, recapitulate the Lengsin temporal and spatial expression patterns. Lengsin function in zebrafish lens formation was examined by antisense morpholino-mediated translation and mRNA splice inhibition. At 72 hpf, the lengsin morphant lenses are reduced in size and exhibit separations within the cortex due to defects in s...Continue Reading

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Citations

Oct 26, 2012·Molecular Biology of the Cell·Julie M HayesJeffrey M Gross
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Jeremy D O'ConnellEdward M Marcotte
May 20, 2011·Developmental Neurobiology·Gaia GestriStephan C F Neuhauss
Dec 4, 2010·Developmental Biology·Rachel K TittleJeffrey M Gross

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