Length control of the injectisome needle requires only one molecule of Yop secretion protein P (YscP).

Proceedings of the National Academy of Sciences of the United States of America
Stefanie WagnerGuy R Cornelis

Abstract

The needle length of the Yersinia spp. injectisome is determined by Yop secretion protein P (YscP), an early substrate of the injectisome itself. There is a linear correlation between the length of YscP and the length of the needle, suggesting that YscP acts as a molecular ruler. However, it is not known whether one single molecule of YscP suffices to control the length of one needle or whether several molecules of YscP are exported in alternation with the needle subunit YscF until the needle length matches the ruler length, which would stop needle growth. To address this question, three different strains expressing simultaneously a short and a long version of YscP were engineered. The experimentally obtained needle length distribution was compared with the distributions predicted by stochastic modeling of the various possible scenarios. The experimental data are compatible with the single ruler model and not with the scenarios involving more than one ruler per needle.

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Citations

Jul 17, 2012·Journal of Bacteriology·Kelly T Hughes
Jul 17, 2012·Journal of Bacteriology·Shin-Ichi Aizawa
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·David Veesler, Christian Cambillau
Nov 28, 2013·PloS One·Basile I M WickyMatteo Dal Peraro
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Daniel H Wee, Kelly T Hughes
Jun 10, 2011·The EMBO Journal·Marc ErhardtKelly T Hughes
Feb 12, 2014·Biochimica Et Biophysica Acta·Brianne J Burkinshaw, Natalie C J Strynadka
Dec 17, 2014·Nature Structural & Molecular Biology·Gregor HageluekenJames H Naismith
Apr 11, 2017·Nature Reviews. Microbiology·Wanyin DengB Brett Finlay
Aug 10, 2013·Cellular Microbiology·Nicola BarisonMichael Kolbe
Nov 22, 2015·The Journal of Biological Chemistry·Julien R C BergeronNatalie C J Strynadka
Nov 8, 2016·Frontiers in Cellular and Infection Microbiology·Meztlli O GaytánBertha González-Pedrajo
Oct 16, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Lele LianJianjun Dai

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