Lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence.

Plant Physiology
Yuichi SakamotoToshitsugu Sato

Abstract

Lentinan is an antitumor product that is purified from fresh Lentinula edodes fruiting bodies. It is a cell wall component, comprising beta-1,3-glucan with beta-1,6-linked branches, which becomes degraded during postharvest preservation as a result of increased glucanase activity. In this study, we used N-terminal amino acid sequence to isolate tlg1, a gene encoding a thaumatin-like (TL) protein in L. edodes. The cDNA clone was approximately 1.0 kb whereas the genomic sequence was 2.1 kb, and comparison of the two indicated that tlg1 contains 12 introns. The tlg1 gene product (TLG1) was predicted to comprise 240 amino acids, with a molecular mass of 25 kD and isoelectric point value of 3.5. The putative amino acid sequence exhibits approximately 40% identity with plant TL proteins, and a fungal genome database search revealed that these TL proteins are conserved in many fungi including the basidiomycota and ascomycota. Transcription of tlg1 was not detected in vegetative mycelium or young and fresh mushrooms. However, transcription increased following harvest. Western-blot analysis demonstrated a rise in TLG1 levels following harvest and spore diffusion. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited beta-1...Continue Reading

References

Oct 1, 1981·Journal of Biochemistry·M ShidaK Matsuda
Jan 1, 1993·Advances in Microbial Physiology·J G Wessels
May 1, 1996·Trends in Microbiology·J M Clarkson, A K Charnley
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·A RietschJ Beckwith
Dec 10, 1998·Plant Physiology·J TrudelA Asselin
Sep 9, 1999·Bioscience, Biotechnology, and Biochemistry·T HiranoH Enei
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·J GrenierA Asselin
Nov 24, 1999·Journal of Agricultural and Food Chemistry·K MinatoH Tsuchida
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·U Kües
Aug 12, 2004·FEBS Letters·Anna BrandazzaPaolo Pelosi
Apr 6, 1984·Science·R G Thorn, G L Barron

❮ Previous
Next ❯

Citations

Nov 11, 2009·Applied Microbiology and Biotechnology·Akira YanoYasuo Yoshida
Apr 28, 2011·Applied Microbiology and Biotechnology·Naotake Konno, Yuichi Sakamoto
Dec 13, 2006·Molecular Plant-microbe Interactions : MPMI·Harold J G MeijerPiet W J de Groot
Oct 4, 2011·Applied and Environmental Microbiology·Yuichi SakamotoNaotake Konno
Feb 18, 2011·BMC Plant Biology·Benjamin PetreSébastien Duplessis
Dec 3, 2014·Fungal Genetics and Biology : FG & B·M A Van der NestJ Stenlid
Aug 21, 2007·Fungal Genetics and Biology : FG & B·Ravit BelaishNir Osherov
Sep 15, 2015·Biochemical and Biophysical Research Communications·Sulamita de Freitas FrancoJorge Maurício Costa Mondego
Sep 18, 2014·Bioscience, Biotechnology, and Biochemistry·Keiichi KobayashiKohtaro Kirimura
May 9, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Markus Künzler
Aug 9, 2017·Chemical Communications : Chem Comm·Zhen QinZhengqiang Jiang
Mar 21, 2017·Applied and Environmental Microbiology·Yuichi SakamotoNaotake Konno
Jun 1, 2019·Journal of the Science of Food and Agriculture·Alberto De IseppiGiovanna Lomolino
Mar 20, 2019·Current Protein & Peptide Science·Carolline de Jesús-PiresAna Maria Benko-Iseppon
Mar 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Krisztina KrizsánLászló G Nagy
May 22, 2019·The Plant Journal : for Cell and Molecular Biology·Vikash KumarEwa J Mellerowicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.