Lentivirus-mediated down-regulation of CK2α inhibits proliferation and induces apoptosis of malignant lymphoma and leukemia cells

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Li JiangLina Quan

Abstract

Casein kinase II subunit alpha (CK2α) is highly expressed in many malignant tumor tissues, including lymphomas and leukemia. To investigate the role of CK2α in cell proliferation and apoptosis of malignant lymphomas and leukemia, 2 lymphoma cell lines and one leukemia cell line were infected with CK2α shRNA lentivirus or negative control shRNA lentivirus, and stably infected cell lines were established. Real-time PCR and Western blot results showed that the mRNA and protein levels of CK2α were significantly reduced in CK2α knockdown cells. The tetrazolium-based colorimetric (MTT) assay found that down-regulation of CK2α inhibited the proliferation of these cells. Flow cytometry analysis showed that inhibition of CK2α induced cell cycle arrest and apoptosis of lymphoma and leukemia cells. In accordance with these, down-regulation of CK2α also reduced the protein levels of proliferating cell nuclear antigen (PCNA), cyclinD1, and bcl-2, and increased the protein expression of bax, cleaved caspase-3, cleaved caspase-9, and cleaved poly(ADP ribose) polymerase (PARP). Moreover, knockdown of CK2α impeded the growth of xenograft tumors in vivo. In summary, our study revealed that CK2α may contribute to the development of malignant lymp...Continue Reading

References

Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Allende, C C Allende
Jan 1, 1997·Progress in Cell Cycle Research·L A Pinna, F Meggio
Jun 5, 2001·Journal of Cellular Biochemistry. Supplement·K AhmedS Tawfic
May 29, 2002·Oncogene·Cunjie ZhangDavid W Litchfield
Dec 14, 2005·Molecular and Cellular Biochemistry·David C SeldinIsabel Dominguez
Feb 10, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jin Seok KimYoo Hong Min
May 22, 2008·Advances in Enzyme Regulation·Kashif A AhmadKhalil Ahmed
Feb 25, 2009·Biochemical and Biophysical Research Communications·Barbara Zschoernig, Ulrich Mahlknecht
Oct 20, 2009·Seminars in Oncology·Samantha M JaglowskiJoseph M Flynn
Sep 25, 2012·Experimental and Molecular Pathology·Md Ashraful IslamDonald M Miller
May 9, 2013·Journal of Cellular and Molecular Medicine·Shulin ZhangLiang You
Jul 19, 2013·Development·Shuhui Lim, Philipp Kaldis
Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Diana UrregoLuis A Pardo
May 17, 2014·International Journal of Cancer. Journal International Du Cancer·Lauren R TerasSusan M Gapstur
Jun 17, 2014·The Cochrane Database of Systematic Reviews·Jonas ZacherNicole Skoetz
Jun 26, 2014·International Journal of Cancer. Journal International Du Cancer·Jun Sang BaeKyu Yun Jang
Dec 20, 2014·Cell Death and Differentiation·S ShaliniS Kumar
Jan 18, 2015·Biochemical and Biophysical Research Communications·Akira MaruyamaTsukasa Seya
Jan 27, 2015·Archives of Toxicology·Waseem Ahmad SiddiquiHaseeb Ahsan
Aug 8, 2015·Mayo Clinic Proceedings·Stephen M Ansell
Jan 26, 2016·Expert Opinion on Drug Safety·Gary H LymanEva Culakova
Jul 21, 2016·Molecular and Cellular Biochemistry·Fatima QaiserKhalil Ahmed
Feb 10, 2017·Journal of Neuro-oncology·Amber L RowseEtty N Benveniste
Nov 18, 2017·Cell Death and Differentiation·Brandon J AubreyAndreas Strasser
Jan 1, 2018·Cell Death and Differentiation·Brandon J AubreyAndreas Strasser

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