Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

Experimental & Molecular Medicine
Jia-Lin SongCun-Yi Fan

Abstract

Our study aims to explore the effects of lentivirus-mediated microRNA-124 (miR-124) gene-modified bone marrow mesenchymal stem cell (BMSC) transplantation on the repair of spinal cord injury (SCI) in rats. BMSCs were isolated from the bone marrow of rats. The target gene miR-124 was identified using a luciferase-reporter gene assay. Seventy-two rats were selected for construction of the SCI model, and the rats were randomly divided into the blank group, sham group, SCI group, negative control (NC) group, overexpressed miR-124 group and si-PDXK group. The mRNA expression of miR-124 and the mRNA and protein expression of pyridoxal kinase (PDXK) were detected by quantitative real-time polymerase chain reaction and western blotting. The locomotor capacity of the rats was evaluated using the Basso, Beattie and Bresnahan (BBB) scale. Brdu, neuron-specific enolase (NSE), neurofilament (NF) and microtubule-associated protein 2 (MAP2) were detected using immunohistochemistry. The expression levels of thyrotropin-releasing hormone (TRH), prostacyclin (PGI2) and gangliosides (GM) were measured using an enzyme-linked immunosorbent assay. PDXK was identified as the target gene of miR-124. The overexpressed miR-124 group exhibited higher miR...Continue Reading

References

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Jul 30, 2016·Journal of Cellular Biochemistry·Joanna CzarneckaKatarzyna Roszek

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Citations

Feb 27, 2019·Genetic Testing and Molecular Biomarkers·Di HuXinguang Qiu
Feb 23, 2019·Acta neurologica Belgica·Fang Li, Mou-Wang Zhou
May 1, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ping SunKe-Jie Yin
Dec 12, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Tao YuYunzhen Chen
May 17, 2019·International Journal of Molecular Sciences·Marta TapparoGiovanni Camussi
Sep 13, 2020·The Journal of Comparative Neurology·Jun ChenZhongtao Liu
Feb 10, 2021·Cell Transplantation·Liyi HuangQuan Wei
Apr 23, 2021·Cellular and Molecular Neurobiology·Jinying XuGuangfan Chi

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Datasets Mentioned

BETA
NM
GM

Methods Mentioned

BETA
PCR
transfection
in vitro transcription
Assay
electrophoresis
ELISA

Software Mentioned

SPSS
Targetscan
ImageQuant 350
ImageQuant

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