Lentivirus-mediated RNAi knockdown of NUPR1 inhibits human nonsmall cell lung cancer growth in vitro and in vivo.

The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Xiaotong GuoYukuan Feng

Abstract

NUPR1 (nuclear protein 1) was found to play a key role in the development of several malignancies including pancreas, breast, and prostate cancers. However, the functional role of NUPR1 in nonsmall cell lung cancer (NSCLC) progression and development is little known. Here, lentivirus-mediated small interfering RNA (siRNA) was employed to downregulate endogenous NUPR1 expression to study the function of NUPR1 in growth of nonsmall cell lung cancer. A lentivirus-mediated RNAi technology was used to specifically knock down the expression of NUPR1 in H1299 cells. Quantitative real-time reverse transcriptase polymerase chain reaction, flow cytometry, western blot and cell count assays were studied to characterize NUPR1 expression in vitro. Furthermore, nonsmall cell lung cancer xenograft models in nude mice were established to investigate whether knockdown of NUPR1 reduces the tumor growth in vivo. We found that downregulation of NUPR1 expression significantly inhibited nonsmall cell lung cancer H1299 cells proliferation and colony formation in vitro. Moreover, the specific downregulation of NUPR1 arrested cells in G0 phase of cell cycle and increased apoptosis rate. Silencing of NUPR1 also suppressed tumor growth by tail vein injec...Continue Reading

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Citations

Jul 9, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jing HuYukuan Feng
Apr 12, 2014·Journal of Gastroenterology and Hepatology·Javed AkhtarZhi Ping Zhang
Jul 22, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Chun-Juan WangRong-Hua Tang
Jun 8, 2015·Apoptosis : an International Journal on Programmed Cell Death·Shouyin DiXiaolong Yan
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Mar 29, 2019·The Journal of Clinical Investigation·Patricia Santofimia-CastañoJuan Iovanna
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Nov 2, 2019·Cellular and Molecular Life Sciences : CMLS·Patricia Santofimia-CastañoJuan Iovanna
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Nov 20, 2018·Scientific Reports·Patricia Santofimia-CastañoJuan Iovanna
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Aug 8, 2021·Cancers·Can HuangJuan Iovanna
Oct 3, 2021·Cell Death Discovery·Can HuangJuan Iovanna

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