Leonurine protects cardiac function following acute myocardial infarction through anti‑apoptosis by the PI3K/AKT/GSK3β signaling pathway

Molecular Medicine Reports
Lin XuHongling Zhu

Abstract

Leonurine is a compound derived from Herba leonuri, which has been reported to protect cardiac tissue against ischemic injury via antioxidant and anti‑apoptosis effects. The present study investigated whether these effects may be applied to acute myocardial infarction (MI) and examined the underlying mechanisms of leonurine treatment. A rat model of MI was induced by coronary artery ligation. Leonurine was administered at 15 mg/kg/day by oral gavage following the onset of MI. Rats in the sham group and the saline group were administered with an equal volume of saline. Echocardiography, Masson's trichrome staining, and terminal‑deoxynucleotidyl transferase‑mediated dUTP nick end labeling assays were performed 28 days post MI. The expression of B‑cell lymphoma‑2 and Bax were assessed by western blot analysis and reverse transcription‑quantitative polymerase chain reaction. Phosphoinositide 3‑kinase (PI3K), protein kinase B and glycogen synthase kinase‑3β (GSK3β) protein expression were investigated by western blot analysis. Leonurine significantly alleviated collagen deposition and MI size, inhibited cell apoptosis and improved myocardial function. This was accompanied by significantly increased levels of phosphorylated (p)‑PI3K,...Continue Reading

References

Mar 4, 2000·Circulation Research·A Haunstetter, S Izumo
Feb 6, 2002·The Journal of Clinical Investigation·Cornel BadorffStefanie Dimmeler
Aug 21, 2003·Biochemical and Biophysical Research Communications·Anthony F TramontanoNabil El-Sherif
Dec 10, 2003·Oncogene·Thomas F FrankeChizuru Sugimoto
Mar 17, 2004·Cancer Treatment Reviews·Juan Angel Fresno VaraManuel González-Barón
Apr 27, 2005·Life Sciences·Jian SunYi Zhun Zhu
Sep 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Yi Zhun ZhuPhilip K Moore
Jan 15, 2009·Apoptosis : an International Journal on Programmed Cell Death·Youngil Lee, Asa B Gustafsson
Apr 16, 2009·British Journal of Pharmacology·Geetha Vani RayasamAbhijit Ray
Jul 15, 2009·Clinical and Experimental Pharmacology & Physiology·Xin-Hua LiuYi-Zhun Zhu
Jan 8, 2010·Molecular and Cellular Biochemistry·Claudia WagnerChristof Weinbrenner
Sep 22, 2010·European Journal of Pharmacology·XinHua LiuYiZhun Zhu
Nov 7, 2012·Free Radical Biology & Medicine·Xin-Hua LiuYi-Zhun Zhu
May 28, 2013·BioMed Research International· Yu-Shengyou, Yu Li

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
protein assay

Software Mentioned

ImageJ

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.